概述

  • 产品名称Anti-ADAMTS13抗体
    参阅全部 ADAMTS13 一抗
  • 描述
    兔多克隆抗体to ADAMTS13
  • 特异性ab28274 recognises the metalloproteinase domain of ADAMTS13.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human ADAMTS13.
    (Peptide available as ab41249)

性能

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应用

Our Abpromise guarantee covers the use of ab28274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 190 kDa (predicted molecular weight: 154 kDa).Can be blocked with ADAMTS13 peptide (ab41249). 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. Glycosylation and the abundance of cysteine residues gives ADAMTS 13 an apparent molecular weight of 190 kDa on reduced SDS PAGE gels. Several bands at 110-190 kDa are observed on Western blots, possibly indicating differentially processed ADAMTS 13. Dilution optimised using Chromogenic detection.

靶标

  • 功能Cleaves the vWF multimers in plasma into smaller forms.
  • 组织特异性Plasma. Expressed primarily in liver.
  • 疾病相关Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP) [MIM:274150]; also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever.
  • 序列相似性Contains 2 CUB domains.
    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
    Contains 8 TSP type-1 domains.
  • 结构域The pro-domain is not required for folding or secretion and does not perform the common function of maintening enzyme latency.
    The spacer domain is necessary to recognize and cleave vWF. The C-terminal TSP type-1 and CUB domains may modulate this interaction.
  • 翻译后修饰May contain a C-mannosylation site and O-fucosylation sites in the TSP type-1 domains.
    The precursor is processed by a furin endopeptidase which cleaves off the pro-domain.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A disintegrin and metalloproteinase with thrombospondin motifs 13 antibody
    • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 13 antibody
    • A disintegrin like and metalloprotease with thrombospondin type 1 motif 13 antibody
    • ADAM metallopeptidase with thrombospondin type 1 motif 13 antibody
    • ADAM TS antibody
    • ADAM-TS 13 antibody
    • ADAM-TS13 antibody
    • ADAMTS 13 antibody
    • ADAMTS-13 antibody
    • ADAMTS13 antibody
    • ADAMTS13 protein antibody
    • ATS13_HUMAN antibody
    • C9orf8 antibody
    • TTP antibody
    • Von Willebrand factor cleaving protease antibody
    • von Willebrand factor-cleaving protease antibody
    • vWF cleaving protease antibody
    • vWF CP antibody
    • vWF-cleaving protease antibody
    • vWF-CP antibody
    • vWFCP antibody
    see all

Anti-ADAMTS13 antibody 图像

  • All lanes : Anti-ADAMTS13 antibody (ab28274) at 1 µg/ml

    Lane 1 : Recombinant Human ATS-13 at 0.08 µg
    Lane 2 : Recombinant Human ATS-13 at 0.04 µg
    Lane 3 : Recombinant Human ATS-13 at 0.02 µg


    Predicted band size : 154 kDa

Anti-ADAMTS13 antibody (ab28274)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (hepatocytes)
Permeabilization Yes - 0.1% Triton X-100
Specification hepatocytes
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative Paraformaldehyde
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提交于 Dec 15 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"