概述

  • 产品名称Anti-ADAMTS1抗体
    参阅全部 ADAMTS1 一抗
  • 描述
    兔多克隆抗体to ADAMTS1
  • 特异性Ab39194 recognizes the zymogen of ADAMTS1, and the furin cleaved ADAMTS1, but not the shed form.
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human ADAMTS1 (C terminal). This antibody recognizes the last 28 amino acids of the protein.
    Database link: Q9UHI8

性能

应用

Our Abpromise guarantee covers the use of ab39194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 105 kDa.

靶标

  • 功能Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture.
  • 序列相似性Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
    Contains 3 TSP type-1 domains.
  • 结构域The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻译后修饰The precursor is cleaved by a furin endopeptidase.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A disintegrin and metalloproteinase with thrombospondin motifs 1 antibody
    • ADAM-TS 1 antibody
    • ADAM-TS1 antibody
    • ADAMTS 1 antibody
    • ADAMTS antibody
    • ADAMTS-1 antibody
    • Adamts1 antibody
    • ATS1_HUMAN antibody
    • C3 C5 antibody
    • KIAA1346, antibody
    • METH 1 antibody
    • METH-1 antibody
    see all

Anti-ADAMTS1 antibody 图像

  • ICC/IF image of ab39194 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39194, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab39194 staining ADAMTS1 in Human Placenta (Decidua) tissue sections by IHC-P (Paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with a peroxidase block for 5 minutes at room temperature. Antigen retrieval was by heat mediation in sodium citrate. Samples were incubated with primary antibody (1/50) in antibody diluent for 1 hour. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody.

    See Abreview

Anti-ADAMTS1 antibody (ab39194)参考文献

This product has been referenced in:
  • Chen J  et al. Downregulation of A disintegrin and metallopeptidase with thrombospondin motif type 1 by DNA hypermethylation in human gastric cancer. Mol Med Rep 12:2487-94 (2015). ICC/IF ; Human . Read more (PubMed: 25936341) »
  • Spencer S  et al. a2d-1 Signaling in Nucleus Accumbens Is Necessary for Cocaine-Induced Relapse. J Neurosci 34:8605-11 (2014). Read more (PubMed: 24948814) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Placenta (Decidua))
Specification Placenta (Decidua)
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate
Blocking step Dako Peroxidase block-> Dako Protein block serum-free ready to use as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Ms. Hyangsin Lee

Verified customer

提交于 Feb 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"