概述

  • 产品名称Anti-ADAMTS1抗体
    参阅全部 ADAMTS1 一抗
  • 描述
    兔多克隆抗体to ADAMTS1
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human ADAMTS1.

  • 阳性对照
    • This antibody gave a positive signal in U20S and A549 whole cell lysates. This antibody gave a positive signal in the following formaldehyde fixed cells: HeLa.

性能

应用

Our Abpromise guarantee covers the use of ab113847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture.
  • 序列相似性Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
    Contains 3 TSP type-1 domains.
  • 结构域The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻译后修饰The precursor is cleaved by a furin endopeptidase.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A disintegrin and metalloproteinase with thrombospondin motifs 1 antibody
    • ADAM-TS 1 antibody
    • ADAM-TS1 antibody
    • ADAMTS 1 antibody
    • ADAMTS antibody
    • ADAMTS-1 antibody
    • Adamts1 antibody
    • ATS1_HUMAN antibody
    • C3 C5 antibody
    • KIAA1346, antibody
    • METH 1 antibody
    • METH-1 antibody
    see all

Anti-ADAMTS1 antibody 图像

  • All lanes : Anti-ADAMTS1 antibody (ab113847) at 1 µg/ml

    Lane 1 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 105 kDa
    Observed band size : 105 kDa
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes
  • ICC/IF image of ab113847 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab113847 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-ADAMTS1 antibody (ab113847)参考文献

ab113847 has not yet been referenced specifically in any publications.

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