概述

  • 产品名称Anti-ADAM33抗体
    参阅全部 ADAM33 一抗
  • 描述
    山羊多克隆抗体to ADAM33
  • 特异性This antibody is expected to recognise isoform alpha (NP_079496) and not isoform beta (NP_694882).
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    CQSRRCRKNAFQEL

    , corresponding to Internal sequence amino acids 637-650 of Human ADAM33

性能

应用

Our Abpromise guarantee covers the use of ab36172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明Peptide ELISA: Antibody detection limit dilution 1:64,000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 88 kDa.
    Experiments gave bands at approx 80-90kDa and strong ladder from 26 to 38kDa in Human Prostate lysates after 0.3ug/ml antibody staining. The observation of multiple bands and the 38 kDa band correspond to earlier findings in literature (Powell et al, Am J Respir Cell Mol Biol. 2004 Jul;31(1):13-21; PMID: 14742294).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 组织特异性Expressed in all tissues, except liver, with high expression in placenta, lung, spleen and veins.
    • 疾病相关Genetic variations in ADAM33 are associated with susceptibility to asthma (ASTHMA) [MIM:600807]. The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with weezing due to spasmodic contraction of the bronchi.
    • 序列相似性Contains 1 disintegrin domain.
      Contains 1 EGF-like domain.
      Contains 1 peptidase M12B domain.
    • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 翻译后修饰The precursor is cleaved by a furin endopeptidase.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • A disintegrin and metalloprotease 33 antibody
      • A disintegrin and metalloproteinase domain 33 antibody
      • ADA33_HUMAN antibody
      • ADAM 33 antibody
      • ADAM metallopeptidase domain 33 antibody
      • ADAM33 antibody
      • C20orf153 antibody
      • Disintegrin and metalloproteinase domain-containing protein 33 antibody
      • Disintegrin and reprolysin metalloproteinase family protein antibody
      • DJ964F7.1 antibody
      • DKFZp434K0521 antibody
      • FLJ35308 antibody
      • FLJ36751 antibody
      • Metalloprotease disintegrin antibody
      • MGC149823 antibody
      • MGC71889 antibody
      • PRO1891 antibody
      • UNQ873 antibody
      see all

    Anti-ADAM33 antibody 图像

    • All lanes : Anti-ADAM33 antibody (ab36172) at 0.3 µg/ml

      Lane 1 : Human Prostate lysate (35µg protein in RIPA buffer) with no blocking from immunizing peptide
      Lane 2 : Human Prostate lysate (35µg protein in RIPA buffer) with blocking from immunizimg peptide


      Predicted band size : 88 kDa

    Anti-ADAM33 antibody (ab36172)参考文献

    This product has been referenced in:
    • Kim KE  et al. Expression of ADAM33 is a novel regulatory mechanism in IL-18-secreted process in gastric cancer. J Immunol 182:3548-55 (2009). WB ; Human . Read more (PubMed: 19265133) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"