概述

  • 产品名称Anti-ADAM33抗体
    参阅全部 ADAM33 一抗
  • 描述
    兔多克隆抗体to ADAM33
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Mouse ADAM33.

  • 阳性对照
    • This antibody gave a positive signal in NIH3T3 whole cell lysate as well as the following Mouse tissue lysates: Placenta; Lung; Spleen; Heart. This antibody gave a positive result in IHC in the following FFPE tissue: mouse normal placenta.

性能

应用

Our Abpromise guarantee covers the use of ab113740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 108 kDa (predicted molecular weight: 87 kDa).
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 组织特异性Expressed in all tissues, except liver, with high expression in placenta, lung, spleen and veins.
  • 疾病相关Genetic variations in ADAM33 are associated with susceptibility to asthma (ASTHMA) [MIM:600807]. The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with weezing due to spasmodic contraction of the bronchi.
  • 序列相似性Contains 1 disintegrin domain.
    Contains 1 EGF-like domain.
    Contains 1 peptidase M12B domain.
  • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻译后修饰The precursor is cleaved by a furin endopeptidase.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A disintegrin and metalloprotease 33 antibody
    • A disintegrin and metalloproteinase domain 33 antibody
    • ADA33_HUMAN antibody
    • ADAM 33 antibody
    • ADAM metallopeptidase domain 33 antibody
    • ADAM33 antibody
    • C20orf153 antibody
    • Disintegrin and metalloproteinase domain-containing protein 33 antibody
    • Disintegrin and reprolysin metalloproteinase family protein antibody
    • DJ964F7.1 antibody
    • DKFZp434K0521 antibody
    • FLJ35308 antibody
    • FLJ36751 antibody
    • Metalloprotease disintegrin antibody
    • MGC149823 antibody
    • MGC71889 antibody
    • PRO1891 antibody
    • UNQ873 antibody
    see all

Anti-ADAM33 antibody 图像

  • All lanes : Anti-ADAM33 antibody (ab113740) at 1 µg/ml

    Lane 1 : Mouse Placenta Tissue Lysate
    Lane 2 : Mouse lung normal tissue lysate - total protein (ab29297)
    Lane 3 : Spleen (Mouse) Tissue Lysate
    Lane 4 : Heart (Mouse) Tissue Lysate
    Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 87 kDa
    Observed band size : 108 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa,42 kDa,68 kDa (possible cleavage fragment),80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • IHC image of ADAM33 staining in mouse normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113740, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-ADAM33 antibody (ab113740)参考文献

ab113740 has not yet been referenced specifically in any publications.

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