Anti-ADAM23抗体-细胞质domain (ab28302)
Key features and details
- Rabbit polyclonal to ADAM23
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-ADAM23抗体-细胞质domain
参阅全部 ADAM23 一抗 -
描述
兔多克隆抗体to ADAM23 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide corresponding to Human ADAM23. Corresponding to the cytoplasmic domain of human ADAM23.
Database link: O75077 -
阳性对照
- ICC: PC12 cells. WB: Human brain tissue lysate. Rat cortex tissue lysate. Human brain amygdala tissue lysate (Alzheimer) membrane extract.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 2.9% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28302于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
Use at an assay dependent dilution. Detects a band of approximately 75 kDa (predicted molecular weight: 92 kDa). 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.
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ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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说明 |
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WB
Use at an assay dependent dilution. Detects a band of approximately 75 kDa (predicted molecular weight: 92 kDa). 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. |
ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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相关性
ADAM23 is a non-catalytic metalloprotease-like protein. It is highly expressed in the brain, primarily in the amygdala, caudate nucleus, hypothalamus, thalamus, cerebral cortex and occipital pole, and weakly expressed in the heart. 3 isoforms are produced by alternative splicing, alpha, beta and gamma. ADAM23 may play a role in cell-cell and cell-matrix interactions. -
细胞定位
Single pass type I membrane protein. 3 isoforms produced by alternative splicing, gamma isoform is secreted. -
数据库链接
- Entrez Gene: 8745 Human
- Entrez Gene: 301460 Rat
- Omim: 603710 Human
- SwissProt: O75077 Human
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别名
- A disintegrin and metalloproteinase domain 23 antibody
- ADAM metallopeptidase domain 23 antibody
- Disintegrin and metalloproteinase domain containing protein 23 antibody
see all
图片
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ICC/IF image of ab28302 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28302, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
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All lanes : Anti-ADAM23 antibody - Cytoplasmic domain (ab28302) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Rat Cortex Tissue Lysate
Lane 3 : Human brain amygdala tissue lysate (alzheimer) - membrane extract (ab30118)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 33 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
The bands observed at 75 kDa could potentially be a cleaved form of ADAM23 due to the presence of a signal peptide and propeptide.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab28302 被引用在 2 文献中.
- Li X et al. miR-301a promotes lung tumorigenesis by suppressing Runx3. Mol Cancer 18:99 (2019). PubMed: 31122259
- Yamagata A et al. Structural basis of epilepsy-related ligand-receptor complex LGI1-ADAM22. Nat Commun 9:1546 (2018). PubMed: 29670100