概述

  • 产品名称Anti-ADAM22抗体
    参阅全部 ADAM22 一抗
  • 描述
    兔多克隆抗体to ADAM22
  • 特异性Reacts specifically with human 100 kDa protein. Cross reacts with ADAM 22 protein from mouse, rat and other isoforms.
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from the C terminal domain of human ADAM22.

性能

应用

Our Abpromise guarantee covers the use of ab66497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/5000. Predicted molecular weight: 100 kDa.
ICC/IF 1/1000.
IHC-P 1/2000.

靶标

  • 功能Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein. Involved in regulation of cell adhesion and spreading and in inhibition of cell proliferation. Neuronal receptor for LGI1.
  • 组织特异性Highly expressed in the brain and in some high-grade but not low-grade gliomas. Detected slightly or not at all in other tissues.
  • 序列相似性Contains 1 disintegrin domain.
    Contains 1 EGF-like domain.
    Contains 1 peptidase M12B domain.
  • 翻译后修饰The precursor is cleaved by a furin endopeptidase.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A disintegrin and metalloproteinase domain 22 antibody
    • ADA22_HUMAN antibody
    • ADAM 22 antibody
    • ADAM metallopeptidase domain 22 antibody
    • adam22 antibody
    • and cysteine-rich protein 2 antibody
    • Disintegrin and metalloproteinase domain-containing protein 22 antibody
    • disintegrin-like antibody
    • MDC 2 antibody
    • MDC2 antibody
    • Metalloproteinase disintegrin ADAM22 3 antibody
    • Metalloproteinase like disintegrin like and cysteine rich protein 2 antibody
    • Metalloproteinase-disintegrin ADAM22-3 antibody
    • Metalloproteinase-like antibody
    • MGC149832 antibody
    see all

Anti-ADAM22 antibody 图像

  • ICC/IF image of ab66497 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66497, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Ab66497 staining human normal cerebellum tissue. Staining is localized to cellular membrane-bound.
    Left panel: with primary antibody diluted 1:2000. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-ADAM22 antibody (ab66497)参考文献

ab66497 has not yet been referenced specifically in any publications.

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