The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 82 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
相关性ADAM19, is a member of the ADAM (a disintegrin and metalloprotease-like domain) family. It has been cloned from mouse and human. ADAM19 was first described in muscle cells as a protein with homology to the fertilins (ADAMs 1 and 2). Initial observations indicated a role for ADAM19 in myoblast fusion, similar to sperm-egg fusion aided by ADAMs 1 and 2. Later works describe ADAM19 in the bone, muscle, lung, heart, brain, kidney, and a wide range of tissues.
The Cytoplasmic domain of ADAM19, like ADAMs 9, 12 and 15, contains SH3 ligand domains, which are thought to interact with PKC-d, suggesting specific regulation routes for ADAM19. Also reported is a sequence of ADAM19 lacking the transmembrane and cytoplasmic domains, suggesting that a soluble form is produced.
ADAM-19 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active. Other ADAMs family members (ADAM-10, ADAM-17) have been more thoroughly studied, and are known to play key roles in inflammation, growth factor maturation and release, and a wide range of other functions. The full length ADAM19 sequence codes for a 956 amino acid protein, containing a Type-I transmembrane domain, with a predicted mass is 105 kD. Two shorter sequences have been reported: a 918 amino acid sequence that differs at the carboxyterminal end, and the soluble form, a 538 amino acid version with predicted mass of 59.9 kD. Mouse ADAM19 sequence is 920 AA, predicted at 100.86 kD.
A disintegrin and metalloproteinase domain 19 (meltrin beta) antibody
A disintegrin and metalloproteinase domain 19 antibody
ADAM 19 antibody
ADAM metallopeptidase domain 19 antibody
Disintegrin and metalloproteinase domain containing protein 19 antibody
FKSG 34 antibody
Meltrin beta antibody
Metalloprotease and disintegrin dentritic antigen marker antibody
Metalloprotease disintegrin meltrin beta antibody
MLTN B antibody
Anti-ADAM19 antibody 图像
Western blot - ADAM19 antibody (ab104800)
Anti-ADAM19 antibody (ab104800) at 1/1 dilution + Human fetal muscle lysate at 10 µg
Predicted band size : 82 kDa
Anti-ADAM19 antibody (ab104800)参考文献
This product has been referenced in:
Marballi K et al. Differential neuregulin 1 cleavage in the prefrontal cortex and hippocampus in schizophrenia and bipolar disorder: preliminary findings. PLoS One7:e36431 (2012).
Read more (PubMed: 22590542) »