Anti-ADAM17抗体(ab75181)

概述

  • 产品名称Anti-ADAM17抗体
    参阅全部 ADAM17 一抗
  • 描述
    兔多克隆抗体to ADAM17
  • 特异性ab75181 detects endogenous levels of fragment of activated ADAM17 resulting from cleavage adjacent to Arg215.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    synthesized peptide derived from a region of human ADAM17.

  • 阳性对照
    • extracts from rat liver cells

性能

应用

Our Abpromise guarantee covers the use of ab75181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 93 kDa.
ELISA 1/20000.

靶标

  • 功能Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.
  • 组织特异性Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.
  • 序列相似性Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
  • 结构域Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻译后修饰The precursor is cleaved by a furin endopeptidase.
    Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
    • A disintegrin and metalloproteinase domain 17 antibody
    • ADA17_HUMAN antibody
    • ADAM 17 antibody
    • ADAM metallopeptidase domain 17 antibody
    • ADAM17 antibody
    • ADAM17 protein antibody
    • CD 156b antibody
    • CD156b antibody
    • CD156b antigen antibody
    • CSVP antibody
    • Disintegrin and metalloproteinase domain-containing protein 17 antibody
    • MGC71942 antibody
    • NISBD antibody
    • NISBD1 antibody
    • Snake venom like protease antibody
    • Snake venom-like protease antibody
    • TACE antibody
    • TNF alpha convertase antibody
    • TNF alpha converting enzyme antibody
    • TNF-alpha convertase antibody
    • TNF-alpha-converting enzyme antibody
    • Tumor Necrosis Factor Alpha Converting Enzyme antibody
    see all

Anti-ADAM17 antibody 图像

  • All lanes : Anti-ADAM17 antibody (ab75181) at 1/500 dilution

    Lane 1 : extracts from rat liver cells
    Lane 2 : extracts from rat liver cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 93 kDa
    Observed band size : ~70 kDa (why is the actual band size different from the predicted?)

Anti-ADAM17 antibody (ab75181)参考文献

ab75181 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam regarding ab75181 and ab28233.

Regarding ab75181, I have confirmed with the laboratory that while the specific information regarding the immunogen sequence is proprietary, the amino acid range is available upon...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"