Synthetic peptide corresponding to Human ADAM17. Only the full length ADAM17 contains the cytoplasmic epitope for this antibody.
(Peptide available as
Our Abpromise guarantee covers the use of ab39162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
WB | 1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 93 kDa). 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. Detects a band of approximately 110 kDa in reduced Western blots of conditioned media or cell lysates. (Predicted molecular weight: 93 kDa). Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. | |
IHC-P | Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
ICC/IF | Use at an assay dependent concentration. | |
IP | Use at an assay dependent concentration. |
ab39162 staining ADAM17 in Human pancreatic carcinoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 10% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/50 in PBS) for 1 hour at 25°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody (1/1000).
ab39162 was used to detect mouse ADAM17 transiently overexpressed in Hela cells. Ab39162 was incubated with the cells at 10 µg/ml for 1 hour at 22°C. For further details please refer to the Abreview.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"