ADAM17抗体(ab39162)| Abcam中国

产品名称

Anti-ADAM17抗体
参阅全部 ADAM17 一抗
描述

兔多克隆抗体to ADAM17
宿主

Rabbit
特异性

This antibody recognizes ADAM17, but does not react with other ADAMs.
经测试应用

适用于: WB, IHC-P, ICC/IF, IPmore details
种属反应性

与反应: Rat, Human, Pig
免疫原

Synthetic peptide corresponding to Human ADAM17. Only the full length ADAM17 contains the cytoplasmic epitope for this antibody.
(Peptide available as ab41214)
阳性对照
- WB: Rat vascular smooth muscle cell whole cell lysate. ICC/IF: Human pancreatic carcinoma cells; HeLa cells transiently overexpressing ADAM17. IHC-P: Human colon tissue.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
存储溶液

Preservative: 0.01% Sodium Azide
Constituents: 50% Glycerol
Concentration information loading...
纯度

Immunogen affinity purified
克隆

多克隆
同种型

IgG
研究领域

Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Immunohistochemistry kits
- EXPOSE Rabbit specific HRP/DAB detection IHC Kit (ab80437)

Our Abpromise guarantee covers the use of ab39162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用	Ab评论	说明
WB		1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 93 kDa). 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. Detects a band of approximately 110 kDa in reduced Western blots of conditioned media or cell lysates. (Predicted molecular weight: 93 kDa). Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.
IHC-P		Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF		Use at an assay dependent concentration.
IP		Use at an assay dependent concentration.

功能

Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.
组织特异性

Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.
序列相似性

Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
结构域

Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
翻译后修饰

The precursor is cleaved by a furin endopeptidase.
Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.
细胞定位

Membrane.
Target information above from: UniProt accession P78536 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 6868 Human
- Entrez Gene: 57027 Rat
- Omim: 603639 Human
- SwissProt: P78536 Human
- SwissProt: Q9Z1K9 Rat
- Unigene: 404914 Human
- Unigene: 144585 Rat
别名
- A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
- A disintegrin and metalloproteinase domain 17 antibody
- ADA17_HUMAN antibody
- ADAM 17 antibody
- ADAM metallopeptidase domain 17 antibody
- ADAM17 antibody
- ADAM17 protein antibody
- CD 156b antibody
- CD156b antibody
- CD156b antigen antibody
- CSVP antibody
- Disintegrin and metalloproteinase domain-containing protein 17 antibody
- MGC71942 antibody
- NISBD antibody
- NISBD1 antibody
- Snake venom like protease antibody
- Snake venom-like protease antibody
- TACE antibody
- TNF alpha convertase antibody
- TNF alpha converting enzyme antibody
- TNF-alpha convertase antibody
- TNF-alpha-converting enzyme antibody
- Tumor Necrosis Factor Alpha Converting Enzyme antibody
see all

Western blot - Anti-ADAM17 antibody (ab39162)

Anti-ADAM17 antibody (ab39162) at 1/1000 dilution + Rat vascular smooth muscle cell whole cell lysate

Secondary
Donkey anti-rabbit Horse Radish Peroxidase at 1/2000 dilution

Predicted band size: 93 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ADAM17 antibody (ab39162)This image is courtesy of an anonymous Abreview

ab39162 staining ADAM17 in Human pancreatic carcinoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 10% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/50 in PBS) for 1 hour at 25°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody (1/1000).
See Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ADAM17 antibody - Cytoplasmic domain(ab39162)

Ab39162 staining human normal colon. Staining is localised to membrane.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Immunocytochemistry/ Immunofluorescence - ADAM17 antibody - Cytoplasmic domain (ab39162)Image taken from Abreview submitted in February 2008

ab39162 was used to detect mouse ADAM17 transiently overexpressed in Hela cells. Ab39162 was incubated with the cells at 10 µg/ml for 1 hour at 22°C. For further details please refer to the Abreview.

Recommended WB protocol

This product has been referenced in:

Hurst LA et al. TNFa drives pulmonary arterial hypertension by suppressing the BMP type-II receptor and altering NOTCH signalling. Nat Commun 8:14079 (2017). WB . Read more (PubMed: 28084316) »
Shirakabe K et al. Mechanistic insights into ectodomain shedding: susceptibility of CADM1 adhesion molecule is determined by alternative splicing and O-glycosylation. Sci Rep 7:46174 (2017). Read more (PubMed: 28393893) »