为了使您在Abcam官网的浏览体验更顺畅，请使用最新版本的浏览器比如 Google Chrome
Our Abpromise guarantee covers the use of ab39161 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 93 kDa). 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. The antibody recognizes a 110 kDa band in reduced Western blots of conditioned media or cell lysates, which is rapidly converted to a 55-60 kDa band. Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.|
|ICC/IF||Use a concentration of 10 µg/ml.|
Methanol fixed HeLa cells incubated with ab39161 at 10 µg/ml for 1 hour at 22°C. Clear membrane staining can be seen. For further details please refer to the Abreview.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"