概述

  • 产品名称Anti-ADAM17抗体
    参阅全部 ADAM17 一抗
  • 描述
    兔多克隆抗体to ADAM17
  • 经测试应用适用于: WB, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human ADAM17 aa 807-823.
    Sequence:

    ASFKLQRQNRVDSKETE


    (Peptide available as ab7881)

  • 阳性对照
    • HeLa whole cell lysate, or Jurkat whole cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human pancreas adenocarcinoma.
  • 常规说明TNFa Converting Enzyme.

应用

Our Abpromise guarantee covers the use of ab2051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 93 kDa.Can be blocked with ADAM17 peptide (ab7881). Detects bands of 80-130 kDa bands, which may represent mature protein, precursor, and glycosylated TACE.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use at an assay dependent concentration. PubMed: 19553533

靶标

  • 功能Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.
  • 组织特异性Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.
  • 序列相似性Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
  • 结构域Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻译后修饰The precursor is cleaved by a furin endopeptidase.
    Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
    • A disintegrin and metalloproteinase domain 17 antibody
    • ADA17_HUMAN antibody
    • ADAM 17 antibody
    • ADAM metallopeptidase domain 17 antibody
    • ADAM17 antibody
    • ADAM17 protein antibody
    • CD 156b antibody
    • CD156b antibody
    • CD156b antigen antibody
    • CSVP antibody
    • Disintegrin and metalloproteinase domain-containing protein 17 antibody
    • MGC71942 antibody
    • NISBD antibody
    • NISBD1 antibody
    • Snake venom like protease antibody
    • Snake venom-like protease antibody
    • TACE antibody
    • TNF alpha convertase antibody
    • TNF alpha converting enzyme antibody
    • TNF-alpha convertase antibody
    • TNF-alpha-converting enzyme antibody
    • Tumor Necrosis Factor Alpha Converting Enzyme antibody
    see all

Anti-ADAM17 antibody 图像

  • All lanes : Anti-ADAM17 antibody (ab2051) at 1/500 dilution

    Lane 1 : HeLa whole cell lysate with absence of blocking peptide
    Lane 2 : Jurkat whole cell lysate with absence of blocking peptide
    Lane 3 : HeLa whole cell lysate with ADAM17 peptide (ab7881)
    Lane 4 : Jurkat whole cell lysate with ADAM17 peptide (ab7881)


    Predicted band size : 93 kDa
    80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated ADAM17.
  • ab2051 at 10µg/ml staining ADAM17 in Hela cells by ICC/IF

  • IHC image of ADAM17 staining in Human pancreas adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2051, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Lane 1 : Anti-ADAM17 antibody (ab2051) at 0.5 µg/ml
    Lane 2 : Unrelated antibody of the same subclass as ab2051.

    Lane 1 : Human keratinocytes (HaCaT)- whole cell lysate from 20000 cells
    Lane 2 : Human keratinocytes (HaCaT)- whole cell lysate from 20000 cells

    Secondary
    HRP conjugated goat polyclonal antibody
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 93 kDa
    Observed band size : 100,125 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • All lanes : Anti-ADAM17 antibody (ab2051) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : U937 cell lysate
    Lane 3 : HT1080 cell lysate
    Lane 4 : ES cell lysate
    Lane 5 : L929 cell lysate
    Lane 6 : CHO cell lysate
    Lane 7 : 293T cell lysate
    Lane 8 : ADAM17-overexpressing 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP-conjugated Goat anti-Rabbit polyclonal antibody at 1/4000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 93 kDa
    Observed band size : 93 kDa
    Additional bands at : 78 kDa (possible isoform).

    Exposure time : 15 seconds

    This image is courtesy of an anonymous Abreview.

    See Abreview

  • Immunofluorescence of TACE in HeLa cells using ab2051 at 10 ug/ml.

Anti-ADAM17 antibody (ab2051)参考文献

This product has been referenced in:
  • Cai Z  et al. Activation of cell-surface proteases promotes necroptosis, inflammation and cell migration. Cell Res N/A:N/A (2016). Read more (PubMed: 27444869) »
  • Brooke MA  et al. iRHOM2-dependent regulation of ADAM17 in cutaneous disease and epidermal barrier function. Hum Mol Genet N/A:N/A (2014). Human . Read more (PubMed: 24643277) »

See all 18 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Intestine)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA
Permeabilization No
Specification Intestine
Fixative Formaldehyde
Username

Dr. PIlar Alzuguren

Verified customer

提交于 Aug 10 2016

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10% in TGS)
Sample Mouse Tissue lysate - other (Whole Hippocampus - Cytosolic and Membrane Fractio)
Specification Whole Hippocampus - Cytosolic and Membrane Fractio
Blocking step Li-Cor Block Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C
Username

Dr. Grant Corbett

Verified customer

提交于 Jul 17 2014

Application Western blot
Loading amount 200 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (hepatocytes)
Specification hepatocytes
Treatment 100 U/ml IL-1
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jul 07 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Primary astrocytes)
Loading amount 2 µg
Specification Primary astrocytes
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Apr 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (primary astrocytes)
Specification primary astrocytes
Fixative Paraformaldehyde
Permeabilization Yes - TX100
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Apr 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Various cell lines)
Loading amount 50 µg
Specification Various cell lines
Gel Running Conditions Reduced Denaturing (5-20% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Mar 02 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (breast)
Loading amount 20 µg
Specification breast
Gel Running Conditions Non-reduced Denaturing (4-12% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Feb 10 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human keratinocytes (HaCaT))
Loading amount 20000 cells
Specification Human keratinocytes (HaCaT)
Blocking step Milk as blocking agent for 10 minute(s) · Concentration: 5
Username

Abcam user community

Verified customer

提交于 Aug 14 2006

Thank you for your enquiry. The anti-TACE antibody has been tested in mouse lung, brain, liver, kidney, spleen, and 3T3 cells.

This antibody cross-reacts with mouse and rat. Other species have not been tested.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"