概述

  • 产品名称Anti-ADAM17抗体
    参阅全部 ADAM17 一抗
  • 描述
    山羊多克隆抗体to ADAM17
  • 特异性This antibody is expected to recognise isoform 1 (represented by NP_003174) and not isoform 2 (represented by NP_068604).
  • 经测试应用适用于: IP, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chinese Hamster
  • 免疫原

    Synthetic peptide: LQRQNRVDSKETEC, corresponding to C terminal amino acids 811-824 of Human ADAM17.

  • 阳性对照
    • Hela Lysate
  • 常规说明GenBank Accession Number - NP_003174

性能

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应用

Our Abpromise guarantee covers the use of ab13535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Detects a band of approximately 125-130 kDa (predicted molecular weight: 95 , when glycosylated 130 kDa).Can be blocked with Human ADAM17 peptide (ab23216) or ADAM17 peptide (ab7881).

靶标

  • 功能Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.
  • 组织特异性Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.
  • 序列相似性Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
  • 结构域Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻译后修饰The precursor is cleaved by a furin endopeptidase.
    Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
    • A disintegrin and metalloproteinase domain 17 antibody
    • ADA17_HUMAN antibody
    • ADAM 17 antibody
    • ADAM metallopeptidase domain 17 antibody
    • ADAM17 antibody
    • ADAM17 protein antibody
    • CD 156b antibody
    • CD156b antibody
    • CD156b antigen antibody
    • CSVP antibody
    • Disintegrin and metalloproteinase domain-containing protein 17 antibody
    • MGC71942 antibody
    • NISBD antibody
    • NISBD1 antibody
    • Snake venom like protease antibody
    • Snake venom-like protease antibody
    • TACE antibody
    • TNF alpha convertase antibody
    • TNF alpha converting enzyme antibody
    • TNF-alpha convertase antibody
    • TNF-alpha-converting enzyme antibody
    • Tumor Necrosis Factor Alpha Converting Enzyme antibody
    see all

Anti-ADAM17 antibody 图像

  • Anti-ADAM17 antibody (ab13535) at 0.2 µg/ml + Hela lysate (RIPA buffer, 35µg total protein per lane)

    Predicted band size : 95 , when glycosylated 130 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • HT1080 cells incubated with ab13535 at 10µg/ml for 1 hour at 22°C. Cells were fixed for 20 minutes in 4% PFA in PBS then permeabilised in 0.1% Triton for 4 minutes

  • ab13535 Immunoprecipitate in human HEK293 whole cell lysate. 2 x 10cells were used to prepare lysates and then incubated with primary antibody (diluted 1/250) and matrix (Protein G) for 16 hours at 4°C. For western blotting an Abcam ab28233 Rabbit polyclonal to ADAM17 was used at dilution at 1/5000.

    See Abreview

Anti-ADAM17 antibody (ab13535)参考文献

This product has been referenced in:
  • Li Q  et al. ADAM17 is critical for multipolar exit and radial migration of neuronal intermediate progenitor cells in mice cerebral cortex. PLoS One 8:e65703 (2013). WB ; Mouse . Read more (PubMed: 23755270) »

See 1 Publication for this product

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Application Western blot
Sample Mouse Cell lysate - whole cell (EL4)
Loading amount 100000 cells
Specification EL4
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 May 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HEK293 cells overexpressing ADAM17)
Specification HEK293 cells overexpressing ADAM17
Immuno-precipitation step Protein G
Username

Abcam user community

Verified customer

提交于 Jun 12 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HT1080 Cell line)
Specification HT1080 Cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
Username

Abcam user community

Verified customer

提交于 Jan 02 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (Immunoprecipitate of HT1080 Cells)
Loading amount 100000 cells
Specification Immunoprecipitate of HT1080 Cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Dec 15 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"