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Our Abpromise guarantee covers the use of ab13535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent dilution.|
|ICC/IF||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution. Detects a band of approximately 125-130 kDa (predicted molecular weight: 95 , when glycosylated 130 kDa).Can be blocked with Human ADAM17 peptide (ab23216) or ADAM17 peptide (ab7881).|
HT1080 cells incubated with ab13535 at 10µg/ml for 1 hour at 22°C. Cells were fixed for 20 minutes in 4% PFA in PBS then permeabilised in 0.1% Triton for 4 minutes
ab13535 Immunoprecipitate in human HEK293 whole cell lysate. 2 x 106 cells were used to prepare lysates and then incubated with primary antibody (diluted 1/250) and matrix (Protein G) for 16 hours at 4°C. For western blotting an Abcam ab28233 Rabbit polyclonal to ADAM17 was used at dilution at 1/5000.
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