概述

  • 产品名称Anti-ADAM15抗体
    参阅全部 ADAM15 一抗
  • 描述
    兔多克隆抗体to ADAM15
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cow, Cat, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 540-589 (QPAAPLCLQT ANTRGNAFGS CGRNPSGSYV SCTPRDAICG QLQCQTGRTQ) of human ADAM15 (NP_997079).

  • 阳性对照
    • Human fetal brain lysate

性能

应用

Our Abpromise guarantee covers the use of ab83883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 93 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain.
  • 组织特异性Expressed in colon and small intestine. Expressed in airway smooth muscle and glomerular mesangial cells (at protein level). Ubiquitously expressed. Overexpressed in atherosclerotic lesions. Constitutively expressed in cultured endothelium and smooth muscle. Expressed in chondrocytes. Expressed in airway smooth muscle and glomerular mesangial cells.
  • 序列相似性Contains 1 disintegrin domain.
    Contains 1 EGF-like domain.
    Contains 1 peptidase M12B domain.
  • 结构域The cytoplasmic domain interacts with endophilin I and sorting nexin 9.
    Disintegrin domain binds to integrin alphaV-beta3.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻译后修饰The precursor is cleaved by a furin endopeptidase.
    Phosphorylation increases association with PTKs.
  • 细胞定位Endomembrane system. Cell junction > adherens junction. Cell projection > cilium > flagellum. Cytoplasmic vesicle > secretory vesicle > acrosome. The majority of the protein is localized in a perinuclear compartment which may correspond to the trans-Golgi network or the late endosome. The pro-protein is the major detectable form on the cell surface, whereas the majority of the protein in the cell is processed.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A disintegrin and metalloproteinase domain 15 (metargidin) antibody
    • A disintegrin and metalloproteinase domain 15 antibody
    • ADA15_HUMAN antibody
    • ADAM 15 antibody
    • ADAM metallopeptidase domain 15 antibody
    • Adam15 antibody
    • and cysteine-rich protein 15 antibody
    • Disintegrin and metalloproteinase domain-containing protein 15 antibody
    • disintegrin-like antibody
    • EC 3.4.24. antibody
    • MDC 15 antibody
    • MDC-15 antibody
    • MDC15 antibody
    • Metalloprotease RGD disintegrin protein antibody
    • Metalloproteinase like disintegrin like and cysteine rich protein 15 antibody
    • Metalloproteinase-like antibody
    • Metargidin antibody
    see all

Anti-ADAM15 antibody 图像

  • Anti-ADAM15 antibody (ab83883) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 93 kDa
    Observed band size : 77 kDa (why is the actual band size different from the predicted?)
  • ab83883 staining ADAM15 in brain tissue by Immunohistochemistry.

Anti-ADAM15 antibody (ab83883)参考文献

ab83883 has not yet been referenced specifically in any publications.

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