Anti-ADAM12抗体(ab56366)

概述

  • 产品名称Anti-ADAM12抗体
    参阅全部 ADAM12 一抗
  • 描述
    小鼠单克隆抗体to ADAM12
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: ETLKATKYVE LVIVADNREF QRQGKDLEKV KQRLIEIANH VDKFYRPLNI RIVLVGVEVW NDMDKCSVSQ DPFTSLHEFL DWRKMKLLPR KSHDNAQ, corresponding to amino acids 208-305 of Human ADAM12

性能

应用

Our Abpromise guarantee covers the use of ab56366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
    • 组织特异性Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.
    • 序列相似性Contains 1 disintegrin domain.
      Contains 1 EGF-like domain.
      Contains 1 peptidase M12B domain.
    • 结构域The cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading.
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 翻译后修饰The precursor is cleaved by a furin endopeptidase.
    • 细胞定位Secreted and Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • A disintegrin and metalloproteinase domain 12 antibody
      • ADA12_HUMAN antibody
      • ADAM 12 antibody
      • ADAM metallopeptidase domain 12 antibody
      • ADAM12 antibody
      • Disintegrin and metalloproteinase domain-containing protein 12 antibody
      • MCMP antibody
      • MCMPMltna antibody
      • Meltrin alpha antibody
      • Meltrin-alpha antibody
      • MLTN antibody
      • MLTNA antibody
      • OTTHUMP00000046766 antibody
      see all

    Anti-ADAM12 antibody 图像

    • Western blot against tagged recombinant protein immunogen using ab56366 ADAM12 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    Anti-ADAM12 antibody (ab56366)参考文献

    ab56366 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Cell lysate - other (Mesenchymal stem cell)
    Gel Running Conditions Reduced Denaturing
    Loading amount 20 µg
    Specification Mesenchymal stem cell
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    提交于 Aug 19 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"