概述

  • 产品名称Anti-ADAM12抗体
    参阅全部 ADAM12 一抗
  • 描述
    山羊多克隆抗体to ADAM12
  • 特异性This antibody is expected to recognise both the longer membrane bound form of human ADAM12 and the shorter soluble ADAM12 splice isoform. This antibody does not cross react with other ADAMS.
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    AARPLPVSPARALC

    , corresponding to N terminal amino acids 2-15 of ADAM12.

  • 阳性对照
    • Human heart lysate. Human prostate tissue.

性能

应用

Our Abpromise guarantee covers the use of ab28747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 100 kDa).
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. PubMed: 19213876

靶标

  • 功能Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
  • 组织特异性Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.
  • 序列相似性Contains 1 disintegrin domain.
    Contains 1 EGF-like domain.
    Contains 1 peptidase M12B domain.
  • 结构域The cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻译后修饰The precursor is cleaved by a furin endopeptidase.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A disintegrin and metalloproteinase domain 12 antibody
    • ADA12_HUMAN antibody
    • ADAM 12 antibody
    • ADAM metallopeptidase domain 12 antibody
    • ADAM12 antibody
    • Disintegrin and metalloproteinase domain-containing protein 12 antibody
    • MCMP antibody
    • MCMPMltna antibody
    • Meltrin alpha antibody
    • Meltrin-alpha antibody
    • MLTN antibody
    • MLTNA antibody
    • OTTHUMP00000046766 antibody
    see all

Anti-ADAM12 antibody 图像

  • Anti-ADAM12 antibody (ab28747) at 0.1 µg/ml + Human heart lysate (RIPA buffer, 30µg total protein per lane)

    Predicted band size : 100 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
  • ab28747 at 3.8µg/ml staining ADAM12 in human prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval using citrate pH 6 was performed. AP staining used as detection method.

Anti-ADAM12 antibody (ab28747)参考文献

This product has been referenced in:
  • Estrella C  et al. Role of A disintegrin and metalloprotease-12 in neutrophil recruitment induced by airway epithelium. Am J Respir Cell Mol Biol 41:449-58 (2009). IHC-P ; Human . Read more (PubMed: 19213876) »

See 1 Publication for this product

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