Anti-ADAM12抗体(ab223476)
Key features and details
- Goat polyclonal to ADAM12
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
概述
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产品名称
Anti-ADAM12抗体
参阅全部 ADAM12 一抗 -
描述
山羊多克隆抗体to ADAM12 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human, Pig
预测可用于: Cow -
免疫原
Synthetic peptide corresponding to Human ADAM12 aa 200-300 (internal sequence) (Cysteine residue). (NP_003465.3).
Database link: O43184 -
阳性对照
- WB: Human, mouse, rat and pig heart lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab223476 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223476于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
1 hour primary incubation is recommended for this product. |
说明 |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. -
组织特异性
Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas. -
序列相似性
Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain. -
结构域
The cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻译后修饰
The precursor is cleaved by a furin endopeptidase. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 407229 Cow
- Entrez Gene: 8038 Human
- Entrez Gene: 11489 Mouse
- Entrez Gene: 100157228 Pig
- Entrez Gene: 679837 Rat
- Omim: 602714 Human
- SwissProt: O43184 Human
- SwissProt: Q61824 Mouse
see all -
别名
- A disintegrin and metalloproteinase domain 12 antibody
- ADA12_HUMAN antibody
- ADAM 12 antibody
see all
图片
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Anti-ADAM12 antibody (ab223476) at 0.1 µg/ml + Human heart lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 100 kDa
Observed band size: 100 kDaPrimary incubation was 1 hour.
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All lanes : Anti-ADAM12 antibody (ab223476) at 0.1 µg/ml
Lane 1 : Mouse heart lysate (in RIPA buffer)
Lane 2 : Rat heart lysate (in RIPA buffer)
Lane 3 : Pig heart lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 100 kDa
Observed band size: 60,90 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab223476 被引用在 1 文献中.
- Zhu YN et al. Role of EZH2-mediated H3K27me3 in placental ADAM12-S expression: implications for fetoplacental growth. BMC Med 20:189 (2022). PubMed: 35610640