RabMAb

Anti-ADAM10抗体[EPR5622] (ab124695)

概述

  • 产品名称Anti-ADAM10抗体[EPR5622]
    参阅全部 ADAM10 一抗
  • 描述
    兔单克隆抗体[EPR5622] to ADAM10
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to residues from the C-terminus of Human ADAM10.

  • 阳性对照
    • Jurkat, RAW 264.7, LnCaP and MCF-7 cell lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab124695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 84 kDa.
IP 1/10 - 1/100.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IHC-P.
  • 靶标

    • 功能Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-
      -Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis.
    • 组织特异性Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.
    • 序列相似性Contains 1 disintegrin domain.
      Contains 1 peptidase M12B domain.
    • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 翻译后修饰The precursor is cleaved by a furin endopeptidase.
    • 细胞定位Cell membrane. Endomembrane system. Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.
    • Information by UniProt
    • 数据库链接
    • 别名
      • A disintegrin and metalloprotease domain 10 antibody
      • A disintegrin and metalloproteinase domain 10 antibody
      • AD 10 antibody
      • AD10 antibody
      • ADA10_HUMAN antibody
      • ADAM 10 antibody
      • ADAM metallopeptidase domain 10 antibody
      • ADAM10 antibody
      • CD 156c antibody
      • CD156c antibody
      • CD156c antigen antibody
      • CDw156 antibody
      • disintegrin and metalloproteinase domain containing protein 10 antibody
      • Disintegrin and metalloproteinase domain-containing protein 10 antibody
      • HsT 18717 antibody
      • HsT18717 antibody
      • Kuz antibody
      • Kuzbanian antibody
      • Kuzbanian protein homolog antibody
      • MADM antibody
      • Mammalian disintegrin metalloprotease antibody
      • Mammalian disintegrin-metalloprotease antibody
      see all

    Anti-ADAM10 antibody [EPR5622] 图像

    • Overlay histogram showing HeLa cells stained with ab124695 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124695, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-ADAM10 antibody [EPR5622] (ab124695) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : RAW 264.7 cell lysate
      Lane 3 : LnCaP cell lysate
      Lane 4 : MCF-7 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 84 kDa
      84 kDa band is pro-ADAM10, smaller band at around 65 kDa is mature ADAM10 protein.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-ADAM10 antibody [EPR5622] (ab124695)参考文献

    This product has been referenced in:
    • Yang C  et al. Enhancement of the nonamyloidogenic pathway by exogenous NGF in an Alzheimer transgenic mouse model. Neuropeptides 48:233-8 (2014). WB ; Mouse . Read more (PubMed: 24813062) »
    • Padro CJ  et al. Adrenergic regulation of IgE involves modulation of CD23 and ADAM10 expression on exosomes. J Immunol 191:5383-97 (2013). Read more (PubMed: 24140643) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Cell lysate - whole cell (primary astrocytes)
    Loading amount 1 µg
    Specification primary astrocytes
    Treatment LPS 0.1ug/ml, 6h
    Gel Running Conditions Reduced Denaturing (10% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

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    Verified customer

    提交于 Apr 16 2013

    The Anti-ADAM10 antibody [EPR5622] (ab124695) has been raised against a synthetic peptide taken from within the region 700 to the C-terminus of the human ADAM10 protein (Uniprot reference O14672).

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"