概述

  • 产品名称Anti-ADA2a抗体
    参阅全部 ADA2a 一抗
  • 描述
    小鼠多克隆抗体to ADA2a
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Fusion protein:

    NFCSARTYDHLKKTREEERLKRTMLSEVLQYIQDSSACQQWLRRQAGIDS GLSPSIPMASNSGRRSAPPLNLTG

    , corresponding to amino acids 296/369 of Human ADA2a

  • 常规说明Produced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Constituents: 50% Glycerol
  • 纯度Whole antiserum
  • Primary antibody说明This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab22175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 51 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 相关性Many DNA binding transcriptional activator proteins enhance the initiation rate of RNA polymerase II mediated gene transcription by interacting functionally with the general transcription machinery bound at the basal promoter. Adaptor proteins are usually required for this activation, possibly to acetylate and destabilize nucleosomes, thereby relieving chromatin constraints at the promoter. ADA2a is a transcriptional activator adaptor and has been found to be part of the PCAF histone acetylase complex.
  • 细胞定位Nuclear, Chromosome
  • 数据库链接
  • 别名
    • ADA2 ALPHA antibody
    • ADA2 antibody
    • ADA2 like antibody
    • ADA2 like protein antibody
    • ADA2, yeast, homolog of, alpha antibody
    • ADA2A antibody
    • hADA2 antibody
    • KL04P antibody
    • Tada2l antibody
    • Transcriptional adapter 2 alpha antibody
    • Transcriptional adapter 2 like antibody
    • Transcriptional adaptor 2 (ADA2 homolog, yeast) like antibody
    • Transcriptional adaptor 2 (ADA2, yeast, homolog) like antibody
    • Transcriptional adaptor 2 alpha antibody
    • Transcriptional adaptor 2A antibody
    see all

Anti-ADA2a antibody 图像

  • All lanes : Anti-ADA2a antibody (ab22175) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 51 kDa

Anti-ADA2a antibody (ab22175)参考文献

ab22175 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab22175.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"