概述

  • 产品名称
    Anti-ADA2 beta抗体
  • 描述
    小鼠单克隆抗体to ADA2 beta
  • 经测试应用
    适用于: WB, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Recombinant fragment, corresponding to amino acids 2-111 of Human ADA2 beta

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

性能

应用

Our Abpromise guarantee covers the use of ab57953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P Use a concentration of 1.5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Flow Cyt Use 0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性
    ADA2 beta is a 48 kDA nuclear protein that functions as a transcriptional adaptor protein that affects transcription by altering histone acetyltransferase activity and linking activation factors to basal transcriptional machinery. It coactivates PAX5 dependent transcription together with either GCN5L2 or SMARCA4.
  • 细胞定位
    Nuclear
  • 数据库链接
  • 别名
    • ADA2 beta antibody
    • ADA2 like protein beta antibody
    • ADA2, yeast, homolog of, beta antibody
    • ADA2B antibody
    • MGC21874 protein antibody
    • TAD 2B antibody
    • TAD2 beta antibody
    • TAD2B antibody
    • TADA2B antibody
    • Transcriptional adapter 2 beta antibody
    • transcriptional adaptor 2 (ADA2 homolog, yeast)-beta antibody
    • transcriptional adaptor 2 antibody
    • transcriptional adaptor 2B antibody
    • yeast ADA2 homolog beta antibody
    see all

图片

  • Overlay histogram showing K562 cells stained with ab57953 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57953, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.


  • Predicted band size : 48 kDa
    ADA2B antibody (ab57953) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab57953 (1.5µg/ml) staining ADA2 beta in formalin-fixed paraffin-embedded human stomach sections.

文献

This product has been referenced in:
  • Mohibi S  et al. Acetylation of Mammalian ADA3 Is Required for Its Functional Roles in Histone Acetylation and Cell Proliferation. Mol Cell Biol 36:2487-502 (2016). Read more (PubMed: 27402865) »
  • Sela D  et al. Role for human mediator subunit MED25 in recruitment of mediator to promoters by endoplasmic reticulum stress-responsive transcription factor ATF6a. J Biol Chem 288:26179-87 (2013). Read more (PubMed: 23864652) »

See all 5 Publications for this product

客户评价及客户问答

Thank you for your email. I am issuing you the discount codes for testing ab2977 and ab57953 in yeast. Please see below. I have heard back regarding ab106169 and ab51024 - unfortunately, both will not cross react due to lack of sufficient seq...

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Thank you for contacting us. I have checked our catalog and have gathered the following information: 1. STE12: We don't have antibodies to this target protein in our catalog. 2. TEC1(ROC1): ab2977 has 100% homology - and qualifi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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