概述

  • 产品名称Anti-ADA2 beta抗体
    参阅全部 ADA2 beta 一抗
  • 描述
    小鼠单克隆抗体to ADA2 beta
  • 经测试应用适用于: WB, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Recombinant fragment, corresponding to amino acids 2-111 of Human ADA2 beta

性能

应用

Our Abpromise guarantee covers the use of ab57953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P Use a concentration of 1.5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Flow Cyt Use 0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性ADA2 beta is a 48 kDA nuclear protein that functions as a transcriptional adaptor protein that affects transcription by altering histone acetyltransferase activity and linking activation factors to basal transcriptional machinery. It coactivates PAX5 dependent transcription together with either GCN5L2 or SMARCA4.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • ADA2 beta antibody
    • ADA2 like protein beta antibody
    • ADA2, yeast, homolog of, beta antibody
    • ADA2B antibody
    • MGC21874 protein antibody
    • TAD 2B antibody
    • TAD2 beta antibody
    • TAD2B antibody
    • TADA2B antibody
    • Transcriptional adapter 2 beta antibody
    • transcriptional adaptor 2 (ADA2 homolog, yeast)-beta antibody
    • transcriptional adaptor 2 antibody
    • transcriptional adaptor 2B antibody
    • yeast ADA2 homolog beta antibody
    see all

Anti-ADA2 beta antibody 图像

  • Overlay histogram showing K562 cells stained with ab57953 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57953, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.


  • Predicted band size : 48 kDa
    ADA2B antibody (ab57953) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab57953 (1.5µg/ml) staining ADA2 beta in formalin-fixed paraffin-embedded human stomach sections.

Anti-ADA2 beta antibody (ab57953)参考文献

This product has been referenced in:
  • Mohibi S  et al. Acetylation of Mammalian ADA3 Is Required for Its Functional Roles in Histone Acetylation and Cell Proliferation. Mol Cell Biol 36:2487-502 (2016). Read more (PubMed: 27402865) »
  • Sela D  et al. Role for human mediator subunit MED25 in recruitment of mediator to promoters by endoplasmic reticulum stress-responsive transcription factor ATF6a. J Biol Chem 288:26179-87 (2013). Read more (PubMed: 23864652) »

See all 4 Publications for this product

Product Wall

Thank you for your email. I am issuing you the discount codes for testing ab2977 and ab57953 in yeast. Please see below. I have heard back regarding ab106169 and ab51024 - unfortunately, both will not cross react due to lack of sufficient seq...

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Thank you for contacting us. I have checked our catalog and have gathered the following information: 1. STE12: We don't have antibodies to this target protein in our catalog. 2. TEC1(ROC1): ab2977 has 100% homology - and qualifi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"