概述

  • 产品名称
    Anti-ADA抗体(HRP)
    参阅全部 ADA 一抗
  • 描述
    兔多克隆抗体to ADA (HRP)
  • 偶联物
    HRP
  • 经测试应用
    适用于: WB, ELISA, Dot blot, Electron Microscopymore details
  • 种属反应性
    与反应: Cow
  • 免疫原

    Adenosine Deaminase (Calf spleen).

性能

应用

Our Abpromise guarantee covers the use of ab34651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
Dot blot
Electron Microscopy
  • 应用说明
    Dot: Use at an assay dependent dilution.
    Immunoperoxidase Electron Microscopy: Use at an assay dependent dilution.
    ELISA: 1/20000 - 1/100000.
    This antibody has been assayed against 1.0µg of Adenosine Deaminase in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 104 kDa.
    Suitable for other peroxidase-antibody based assays.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion.
    • 组织特异性
      Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues.
    • 疾病相关
      Defects in ADA are the cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-negative due to adenosine deaminase deficiency (ADASCID) [MIM:102700]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. ADA-SCID is an autosomal recessive form accounting for about 50% of non-X-linked SCIDs. ADA deficiency has been diagnosed in chronically ill teenagers and adults (late or adult onset). Population and newborn screening programs have also identified several healthy individuals with normal immunity who have partial ADA deficiency.
    • 序列相似性
      Belongs to the adenosine and AMP deaminases family.
    • 细胞定位
      Cell membrane. Cell junction. Cytoplasmic vesicle lumen. Cytoplasm. Colocalized with DPP4 at the cell junction in lymphocyte-epithelial cell adhesion.
    • Information by UniProt
    • 别名
      • ada antibody
      • ADA_HUMAN antibody
      • ADA1 antibody
      • Adenosine aminohydrolase antibody
      • Adenosine deaminase antibody
      see all

    实验方案

    文献

    ab34651 has not yet been referenced specifically in any publications.

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