概述

  • 产品名称Anti-ADA抗体(Biotin)
    参阅全部 ADA 一抗
  • 描述
    兔多克隆抗体to ADA (Biotin)
  • 偶联物Biotin
  • 经测试应用适用于: Flow Cyt, Dot blot, ELISA, Immunomicroscopymore details
  • 种属反应性
    与反应: Cow, Human
  • 免疫原

    Adenosine Deaminase from Calf spleen.

  • 常规说明

    10-20 Biotin molecules per rabbit IgG molecule.

性能

应用

Our Abpromise guarantee covers the use of ab34677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. (PMID 20147632)



ab200208-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

This antibody has been assayed against 1.0µg of Adenosine Deaminase in a standard capture ELISA using Peroxidase conjugated streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT.

Immunomicroscopy Use at an assay dependent concentration.

靶标

  • 功能Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion.
  • 组织特异性Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues.
  • 疾病相关Defects in ADA are the cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-negative due to adenosine deaminase deficiency (ADASCID) [MIM:102700]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. ADA-SCID is an autosomal recessive form accounting for about 50% of non-X-linked SCIDs. ADA deficiency has been diagnosed in chronically ill teenagers and adults (late or adult onset). Population and newborn screening programs have also identified several healthy individuals with normal immunity who have partial ADA deficiency.
  • 序列相似性Belongs to the adenosine and AMP deaminases family.
  • 细胞定位Cell membrane. Cell junction. Cytoplasmic vesicle lumen. Cytoplasm. Colocalized with DPP4 at the cell junction in lymphocyte-epithelial cell adhesion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ada antibody
    • ADA_HUMAN antibody
    • ADA1 antibody
    • Adenosine aminohydrolase antibody
    • Adenosine deaminase antibody
    see all

Anti-ADA antibody (Biotin) (ab34677)参考文献

This product has been referenced in:
  • Chou JP  et al. Accelerated aging in HIV/AIDS: novel biomarkers of senescent human CD8+ T cells. PLoS One 8:e64702 (2013). Flow Cyt ; Human . Read more (PubMed: 23717651) »
  • Parish ST  et al. Adenosine deaminase modulation of telomerase activity and replicative senescence in human CD8 T lymphocytes. J Immunol 184:2847-54 (2010). Flow Cyt ; Human . Read more (PubMed: 20147632) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry and your interest in our products. According to the datasheets, ab54969 has not been tested for ELISA (only WB, Flow Cytmetry); whilst the other antibody (ab34677) has been characterized for indirect ELISA system. Unfor...

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Thank you for your patience while I investigated the source of the note on our datasheet that specified the molecular weight of ADA as 104 kDa. The predicted molecular weight should be given as 40.7 kDa, based on the UniProt database entry: ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"