概述

  • 产品名称
    Anti-Acylglycerol Kinase抗体
    参阅全部 Acylglycerol Kinase 一抗
  • 描述
    山羊多克隆抗体to Acylglycerol Kinase
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide:

    CDPRKREQMLTSP

    , corresponding to C terminal amino acids 408-420 of Human Acylglycerol Kinase (NP_060708.1).

  • 阳性对照
    • Lysate from Human Brain (Substantia nigra, Hippocampus)

性能

应用

Our Abpromise guarantee covers the use of ab77266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/16000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth.
    • 组织特异性
      Highly expressed in muscle, heart, kidney and brain.
    • 通路
      Lipid metabolism; glycerolipid metabolism.
    • 序列相似性
      Contains 1 DAGKc domain.
    • 细胞定位
      Mitochondrion membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 2610037M15Rik antibody
      • 6720408I04Rik antibody
      • Acylglycerol kinase mitochondrial antibody
      • Acylglycerol kinase, mitochondrial antibody
      • agk antibody
      • AGK_HUMAN antibody
      • AI465370 antibody
      • FLJ10842 antibody
      • hAGK antibody
      • HsMuLK antibody
      • MuLK antibody
      • Multi substrate lipid kinase antibody
      • Multi-substrate lipid kinase antibody
      • Multiple substrate lipid kinase antibody
      • RGD1562046 antibody
      see all

    图片

    • Anti-Acylglycerol Kinase antibody (ab77266) at 1 µg/ml + Human Brain (Substantia nigra) lysate in RIPA buffer at 35 µg

      Predicted band size : 47 kDa
      Observed band size : 47 kDa
      Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.

    实验方案

    文献

    This product has been referenced in:
    • Abu El-Asrar AM  et al. Expression of bioactive lysophospholipids and processing enzymes in the vitreous from patients with proliferative diabetic retinopathy. Lipids Health Dis 13:187 (2014). Read more (PubMed: 25496321) »
    • Abu El-Asrar AM  et al. Expression of lysophosphatidic acid, autotaxin and acylglycerol kinase as biomarkers in diabetic retinopathy. Acta Diabetol 50:363-71 (2013). Read more (PubMed: 22864860) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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