Customer reviews and FAQs

概述

  • 产品名称
    Anti-Acylglycerol Kinase抗体
    参阅全部 Acylglycerol Kinase 一抗
  • 描述
    山羊多克隆抗体to Acylglycerol Kinase
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide:

    CDPRKREQMLTSP

    , corresponding to C terminal amino acids 408-420 of Human Acylglycerol Kinase (NP_060708.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • Lysate from Human Brain (Substantia nigra, Hippocampus)

性能

应用

Our Abpromise guarantee covers the use of ab77266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/16000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • 靶标

    • 功能
      Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth.
    • 组织特异性
      Highly expressed in muscle, heart, kidney and brain.
    • 通路
      Lipid metabolism; glycerolipid metabolism.
    • 序列相似性
      Contains 1 DAGKc domain.
    • 细胞定位
      Mitochondrion membrane.
    • Information by UniProt
    • 数据库链接
      • 别名
        • 2610037M15Rik antibody
        • 6720408I04Rik antibody
        • Acylglycerol kinase mitochondrial antibody
        • Acylglycerol kinase, mitochondrial antibody
        • agk antibody
        • AGK_HUMAN antibody
        • AI465370 antibody
        • FLJ10842 antibody
        • hAGK antibody
        • HsMuLK antibody
        • MuLK antibody
        • Multi substrate lipid kinase antibody
        • Multi-substrate lipid kinase antibody
        • Multiple substrate lipid kinase antibody
        • RGD1562046 antibody
        see all

      Anti-Acylglycerol Kinase antibody 图像

      • Western blot - Acylglycerol Kinase antibody (ab77266)
        Western blot - Acylglycerol Kinase antibody (ab77266)
        Anti-Acylglycerol Kinase antibody (ab77266) at 1 µg/ml + Human Brain (Substantia nigra) lysate in RIPA buffer at 35 µg

        Predicted band size : 47 kDa
        Observed band size : 47 kDa
        Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.

      实验方案

      Anti-Acylglycerol Kinase antibody (ab77266)参考文献

      This product has been referenced in:
      • Abu El-Asrar AM  et al. Expression of lysophosphatidic acid, autotaxin and acylglycerol kinase as biomarkers in diabetic retinopathy. Acta Diabetol 50:363-71 (2013). Read more (PubMed: 22864860) »

      See 1 Publication for this product

      发表研究结果有使用 ab77266？请让我们知道，以便我们可以引用本数据表中的参考文章。

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