Anti-Acylglycerol Kinase抗体(ab77266)
- 数据表
- 具体的参考文献 (2)
- 实验方案
概述
-
产品名称Anti-Acylglycerol Kinase抗体
参阅全部 Acylglycerol Kinase 一抗 -
描述山羊多克隆抗体to Acylglycerol Kinase
-
宿主Goat
-
经测试应用适用于: WB, ELISAmore details
-
种属反应性与反应: Human
预测可用于: Mouse
-
免疫原
Synthetic peptide:
CDPRKREQMLTSP
, corresponding to C terminal amino acids 408-420 of Human Acylglycerol Kinase (NP_060708.1). -
阳性对照
- Lysate from Human Brain (Substantia nigra, Hippocampus)
性能
-
形式Liquid
-
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
-
存储溶液Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
纯度Immunogen affinity purified
-
纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
-
克隆多克隆
-
同种型IgG
-
研究领域
相关产品
-
Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab77266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| ELISA |
应用说明
Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/16000.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
-
功能Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth.
-
组织特异性Highly expressed in muscle, heart, kidney and brain.
-
通路Lipid metabolism; glycerolipid metabolism.
-
序列相似性Contains 1 DAGKc domain.
-
细胞定位Mitochondrion membrane.
- Information by UniProt
-
数据库链接
- Entrez Gene: 55750 Human
- Entrez Gene: 69923 Mouse
- Omim: 610345 Human
- SwissProt: Q53H12 Human
- SwissProt: Q9ESW4 Mouse
- Unigene: 699361 Human
- Unigene: 32840 Mouse
-
别名
- 2610037M15Rik antibody
- 6720408I04Rik antibody
- Acylglycerol kinase mitochondrial antibody
see all
图片
-
Western blot - Anti-Acylglycerol Kinase antibody (ab77266)Anti-Acylglycerol Kinase antibody (ab77266) at 1 µg/ml + Human Brain (Substantia nigra) lysate in RIPA buffer at 35 µg
Predicted band size: 47 kDa
Observed band size: 47 kDa
Additional bands at: 22 kDa. We are unsure as to the identity of these extra bands.
Both bands were successfully blocked by pre-incubation with the immunizing peptide.
文献
This product has been referenced in:
- Abu El-Asrar AM et al. Expression of bioactive lysophospholipids and processing enzymes in the vitreous from patients with proliferative diabetic retinopathy. Lipids Health Dis 13:187 (2014). Read more (PubMed: 25496321) »
- Abu El-Asrar AM et al. Expression of lysophosphatidic acid, autotaxin and acylglycerol kinase as biomarkers in diabetic retinopathy. Acta Diabetol 50:363-71 (2013). Read more (PubMed: 22864860) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"