Anti-AcV5 tag抗体[AcV5] (ab49581)

概述

  • 产品名称Anti-AcV5 tag抗体[AcV5]
  • 描述
    小鼠单克隆抗体[AcV5] to AcV5 tag
  • 特异性This antibody recognizes a nine amino acid residue tag (SWKDASGWS) of the baculovirus Autographa californica GP64 envelope fusion protein. Proteins tagged with this AcV5 peptide are functional in both dicots and monocots plants and show no cross reactivity across many plant species.
  • 经测试应用适用于: IP, ICC, Dot blotmore details
  • 种属反应性
    Reacts with
  • 免疫原

    AcNPV extracellular nonocculeded virus (NOV)

  • 阳性对照
    • Extracts of Sf9 cells infected with baculovirus.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 克隆单克隆
  • 克隆编号AcV5
  • 骨髓瘤Sp2/0-Ag14
  • 同种型IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab49581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
Dot blot Use a concentration of 0.5 - 1 µg/ml.

靶标

  • 相关性Epitope tagged fusion proteins such as AcV5 tag are widely used in protein analysis of biological samples (e.g. plant cell extracts). Antibodies specific for the tag are a valuable means for the detection of the protein. AcV5 recognizes a nine amino acid residue tag of baculovirus Autographa californica GP64 envelope fusion protein (efp). GP64 gene open reading frame encodes a protein of 509 amino acids with a molecular weight of 58 kDa. The protein contains N-linked glycosylation sites and hydrophobic N- and C-termini, characteristic of signal and membrane anchor motifs found in envelope glycoproteins. The GP64 protein is present early (6 hr) post infection and accumulates in the infected cell moving to the periphery later.

Anti-AcV5 tag antibody [AcV5] (ab49581)参考文献

ab49581 has not yet been referenced specifically in any publications.

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