重组
RabMAb

Anti-ACTN3抗体[EP2531Y] (ab68204)

概述

  • 产品名称
    Anti-ACTN3抗体[EP2531Y]
    参阅全部 ACTN3 一抗
  • 描述
    兔单克隆抗体[EP2531Y] to ACTN3
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IF, WB, IHC-P, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues on the N-terminus of human ACTN3.

  • 阳性对照
    • Human skeletal muscle lysate and human muscle tissue
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab68204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
WB 1/5000 - 1/10000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 功能
      F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
    • 组织特异性
      Expressed only in a subset of type 2 skeletal muscle fibers.
    • 序列相似性
      Belongs to the alpha-actinin family.
      Contains 1 actin-binding domain.
      Contains 2 CH (calponin-homology) domains.
      Contains 2 EF-hand domains.
      Contains 4 spectrin repeats.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Actinin alpha 3 antibody
      • actinin, alpha antibody
      • ACTN 3 antibody
      • ACTN3 antibody
      • ACTN3_HUMAN antibody
      • Alpha actinin skeletal muscle antibody
      • Alpha actinin skeletal muscle isoform 3 antibody
      • Alpha-Actinin 3 antibody
      • Alpha-actinin skeletal muscle isoform 3 antibody
      • Alpha-actinin-3 antibody
      • F actin cross linking protein antibody
      • F-actin cross-linking protein antibody
      • MGC117002 antibody
      • MGC117005 antibody
      • OTTHUMP00000219049 antibody
      • OTTHUMP00000219054 antibody
      see all

    图片

    • Anti-ACTN3 antibody [EP2531Y] (ab68204) at 1/10000 dilution + human skeletal muscle lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 103 kDa
      Observed band size: 103 kDa

    • ab68204 staining ACTN3 in human pluripotent stem cell derived cardiomyocyte by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 5% serum for 15 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor® 568-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

      See Abreview

    • Immunohistochemical analysis of paraffin-embedded human muscle tissue using ab68204 at 1/100-1/250 dilution.

    文献

    ab68204 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Filter by Application

    Filter by Species

    Filter by Ratings

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Heart)
    Permeabilization
    Yes - Tween-20
    Specification
    Heart
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Aug 22 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Cell (skeletal muscle/frozen sections, human, rat and mo)
    Permeabilization
    Yes - 0,1 % Triton X-100 in PBS
    Specification
    skeletal muscle/frozen sections, human, rat and mo
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Histofix/Histolab/Sweden
    Username

    Satu Koskinen

    Verified customer

    提交于 Aug 01 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Skeletal Muscle)
    Gel Running Conditions
    Reduced Denaturing (4%-12%)
    Loading amount
    10 µg
    Specification
    Skeletal Muscle
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Jul 25 2016

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human pluripotent stem cell derived cardiomyocyte)
    Permeabilization
    Yes - saponin
    Specification
    Human pluripotent stem cell derived cardiomyocyte
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Apr 21 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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