概述

  • 产品名称Anti-ACTN3抗体
    参阅全部 ACTN3 一抗
  • 描述
    兔多克隆抗体to ACTN3
  • 经测试应用适用于: ELISA, WB, IHC-Frmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from N-terminus of human ACTN3.

  • 阳性对照
    • Extracts from HT-29 cells

性能

应用

Our Abpromise guarantee covers the use of ab65067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
IHC-Fr Use at an assay dependent concentration. PubMed: 23174654

靶标

  • 功能F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
  • 组织特异性Expressed only in a subset of type 2 skeletal muscle fibers.
  • 序列相似性Belongs to the alpha-actinin family.
    Contains 1 actin-binding domain.
    Contains 2 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
    Contains 4 spectrin repeats.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Actinin alpha 3 antibody
    • actinin, alpha antibody
    • ACTN 3 antibody
    • ACTN3 antibody
    • ACTN3_HUMAN antibody
    • Alpha actinin skeletal muscle antibody
    • Alpha actinin skeletal muscle isoform 3 antibody
    • Alpha-Actinin 3 antibody
    • Alpha-actinin skeletal muscle isoform 3 antibody
    • Alpha-actinin-3 antibody
    • F actin cross linking protein antibody
    • F-actin cross-linking protein antibody
    • MGC117002 antibody
    • MGC117005 antibody
    • OTTHUMP00000219049 antibody
    • OTTHUMP00000219054 antibody
    see all

Anti-ACTN3 antibody 图像

  • All lanes : Anti-ACTN3 antibody (ab65067) at 1/500 dilution

    Lane 1 : extracts from HT-29 cells
    Lane 2 : extracts from HT-29 cells with immunizing peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 103 kDa
    Observed band size : 103 kDa

Anti-ACTN3 antibody (ab65067)参考文献

This product has been referenced in:
  • Moncman CL  et al. Development transitions of thin filament proteins in rat extraocular muscles. Exp Cell Res : (2012). WB, IHC-Fr ; Rat . Read more (PubMed: 23174654) »

See 1 Publication for this product

Product Wall

Thank you for contacting Abcam regarding ab65067. I have confirmed with the laboratory that the immunogen sequence homology is 75% with ACTN1, 83% with ACTN2, 75% with ACTN4. We have not specifically tested if ab65067 will cross react with these pr...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"