Anti-Activin Receptor Type IIB抗体(ab76940)


  • 产品名称Anti-Activin Receptor Type IIB抗体
    参阅全部 Activin Receptor Type IIB 一抗
  • 描述
    小鼠单克隆抗体to Activin Receptor Type IIB
  • 经测试应用适用于: WB, ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Sheep, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Macaque Monkey
  • 免疫原

    Recombinant fragment with proprietary tag: RGEAETRECI YYNANWELER TNQSGLERCE GEQDKRLHCY ASWANSSGTI ELVKKGCWLD DFNCYDRQEC VATEENPQVY FCCCEGNFCN ERFTHLPEAG, corresponding to amino acids 21-120 of Human Activin Receptor Type IIB(NP_001097)

  • 阳性对照
    • Lysate from 293T cells transfected with Activin Receptor Type IIB



Our Abpromise guarantee covers the use of ab76940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 58 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • 功能On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin A, activin B and inhibin A.
  • 疾病相关Defects in ACVR2B are the cause of visceral heterotaxy autosomal type 4 (HTX4) [MIM:613751]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy type 4 include dextrocardia, right aortic arch and a right-sided spleen, anomalies of the inferior and the superior vena cava, atrial ventricular canal defect with dextro-transposed great arteries, pulmonary stenosis, polysplenia and midline liver.
  • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 protein kinase domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activin A receptor type IIB antibody
    • Activin receptor type 2B antibody
    • Activin receptor type IIB antibody
    • Activin receptor type-2B antibody
    • ActR IIB antibody
    • ACTR-IIB antibody
    • ActRIIB antibody
    • ACVR 2B antibody
    • ACVR2B antibody
    • AVR2B_HUMAN antibody
    • HTX4 antibody
    • MGC116908 antibody
    see all

Anti-Activin Receptor Type IIB antibody 图像

  • Anti-Activin Receptor Type IIB antibody (ab76940) at 1/500 dilution + immunogen (GST-tagged 100aa recombinant fragment) at 0.2 µg

    Goat anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution

    Predicted band size : 58 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing HeLa cells stained with ab76940 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76940, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • All lanes : Anti-Activin Receptor Type IIB antibody (ab76940) at 1/500 dilution

    Lane 1 : Activin Receptor Type IIB transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 58 kDa

Anti-Activin Receptor Type IIB antibody (ab76940)参考文献

This product has been referenced in:
  • Perron JC & Dodd J Inductive specification and axonal orientation of spinal neurons mediated by divergent bone morphogenetic protein signaling pathways. Neural Dev 6:36 (2011). Read more (PubMed: 22085733) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Odyssey Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Sample Human Cell (LNCaP Human Cell Line)
Specification LNCaP Human Cell Line
Permeabilization Yes - Ethanol
Fixative Paraformaldehyde


Verified customer

提交于 Mar 25 2015

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (12)
Sample Mouse Tissue lysate - whole (Testis)
Specification Testis
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C

Abcam user community

Verified customer

提交于 Oct 21 2014

Merci de nous avoir contactés.

Suite à notre conversation du 7 Novembre, j’ai demandé au laboratoire des explications pour les images du western blot pour ab76940. Puisque les deux images étaient contradicto...

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Suite à notre conversation téléphonique je vais demander au laboratoire des explications pour les images de western blot pour ab76940. En attendant leur réponse, je vous envoie le code pour l’offre de test pour ab12854...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (C2C12 mouse myoblast and C57 muscle extract)
Loading amount 40 µg
Specification C2C12 mouse myoblast and C57 muscle extract
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Mr. Thomas Roberts

Verified customer

提交于 Jul 17 2009