Anti-Activin Receptor Type IIB抗体(ab10596)

概述

  • 产品名称Anti-Activin Receptor Type IIB抗体
    参阅全部 Activin Receptor Type IIB 一抗
  • 描述
    山羊多克隆抗体to Activin Receptor Type IIB
  • 特异性Based on ELISA, this antibody exhibits approximately 15 % cross-reactivity with recombinant human activin receptor IA. In immunoblotting, this antibody shows no cross-reactivity with recombinant human activin receptor IIA.
  • 经测试应用适用于: IHC-P, Indirect ELISA, WB, Neutralisingmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment (Human).

  • 常规说明


    Activin, a disulfide-linked homodimeric protein is secreted by Sertoli cells in the testis and granulosa cells in the ovary. In early studies, this peptide was thought to be an inhibin and not recognized as a unique compound. Activins and inhibins are members of the TGF-beta superfamily due to amino acid homology with respect to the conservation of 7 of the 9 cysteine residues common to all TGF-beta forms. Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. Five beta subunits have been cloned (mammalian beta A, beta B, beta C, beta E, and Xenopus beta D). The activin/inhibin nomenclature reflects the subunit composition of the proteins: activin A (beta A-beta A), activin B (beta B-beta B), activin AB (beta B-beta A), inhibin A (alpha-beta A) abd inhibin B (alpha-beta B). Activins have a wide range of biological activities including mesoderm induction neural cell differentiation, bone remodeling, hematopoiesis, and reproductive physiology. Activins are also involved in growth and differentiation of several tissues from different species. This protein also plays a key role in the production and regulation of hormones such as FSH, LH, GnRH, and ACTH. Activin influences erythropoiesis and the potentiation of erythroid colony formation, oxytocin secretion, paracrine, and autocrine regulation. Similar to other TGF-beta family members, activins exert their biological activities through the effects of the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated type I and type II receptors. Activin type I and type II receptors are distinguished by the level of sequence homology of their kinase domains and other structural and functional features. To date, seven type I and five type II activin receptors have been cloned from mammals, including activin receptor IA, activin receptor IIA, activin receptor IB, and activin receptor IIB. In addition, two splice variants of activin receptor IIA and five splice variants of activin receptor IIB have been reported. Type I activin receptors do not bind directly to activin but will associate with the type II receptor-activin complex and initiate signal transduction. Recombinant soluble activin type II receptors bind activin with high affinity and are potent activin antagonists. Activin type II receptors are highly conserved and will also bind inhibin, BMP2, and BMP7 with lower affinities. Human, mouse, and rat type II activin receptors share 98 % homology.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 5% Trehalose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • Primary antibody说明Activin, a disulfide-linked homodimeric protein is secreted by Sertoli cells in the testis and granulosa cells in the ovary. In early studies, this peptide was thought to be an inhibin and not recognized as a unique compound. Activins and inhibins are members of the TGF-beta superfamily due to amino acid homology with respect to the conservation of 7 of the 9 cysteine residues common to all TGF-beta forms. Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. Five beta subunits have been cloned (mammalian beta A, beta B, beta C, beta E, and Xenopus beta D). The activin/inhibin nomenclature reflects the subunit composition of the proteins: activin A (beta A-beta A), activin B (beta B-beta B), activin AB (beta B-beta A), inhibin A (alpha-beta A) abd inhibin B (alpha-beta B). Activins have a wide range of biological activities including mesoderm induction neural cell differentiation, bone remodeling, hematopoiesis, and reproductive physiology. Activins are also involved in growth and differentiation of several tissues from different species. This protein also plays a key role in the production and regulation of hormones such as FSH, LH, GnRH, and ACTH. Activin influences erythropoiesis and the potentiation of erythroid colony formation, oxytocin secretion, paracrine, and autocrine regulation. Similar to other TGF-beta family members, activins exert their biological activities through the effects of the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated type I and type II receptors. Activin type I and type II receptors are distinguished by the level of sequence homology of their kinase domains and other structural and functional features. To date, seven type I and five type II activin receptors have been cloned from mammals, including activin receptor IA, activin receptor IIA, activin receptor IB, and activin receptor IIB. In addition, two splice variants of activin receptor IIA and five splice variants of activin receptor IIB have been reported. Type I activin receptors do not bind directly to activin but will associate with the type II receptor-activin complex and initiate signal transduction. Recombinant soluble activin type II receptors bind activin with high affinity and are potent activin antagonists. Activin type II receptors are highly conserved and will also bind inhibin, BMP2, and BMP7 with lower affinities. Human, mouse, and rat type II activin receptors share 98 % homology.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent dilution. PubMed: 19638618
Indirect ELISA Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 58 kDa. ~1 ng/lane under non-reducing and reducing conditions.
Neutralising Use a concentration of 1 - 3 µg/ml. The antibody will block 50% of the binding of 30 ng/ml recombinant human activin A to immobilized recombinant human activin receptor IIB/Fc chimera (100 µL of 50 µg/ml solution coating each well) in a functional ELISA assay. ab10596 has not been optimized for cell/tissue culture conditions.

靶标

  • 功能On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin A, activin B and inhibin A.
  • 疾病相关Defects in ACVR2B are the cause of visceral heterotaxy autosomal type 4 (HTX4) [MIM:613751]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy type 4 include dextrocardia, right aortic arch and a right-sided spleen, anomalies of the inferior and the superior vena cava, atrial ventricular canal defect with dextro-transposed great arteries, pulmonary stenosis, polysplenia and midline liver.
  • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 protein kinase domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activin A receptor type IIB antibody
    • Activin receptor type 2B antibody
    • Activin receptor type IIB antibody
    • Activin receptor type-2B antibody
    • ActR IIB antibody
    • ACTR-IIB antibody
    • ActRIIB antibody
    • ACVR 2B antibody
    • ACVR2B antibody
    • AVR2B_HUMAN antibody
    • HTX4 antibody
    • MGC116908 antibody
    see all

Anti-Activin Receptor Type IIB antibody 图像

  • ACVR2B staining was mainly localized at the membrane level of the epithelial cells in the normal tissues with a clear down-regulation in tumoral epithelia.

Anti-Activin Receptor Type IIB antibody (ab10596)参考文献

This product has been referenced in:
  • Babel I  et al. Identification of tumor-associated autoantigens for the diagnosis of colorectal cancer in serum using high density protein microarrays. Mol Cell Proteomics 8:2382-95 (2009). IHC-P ; Human . Read more (PubMed: 19638618) »

See 1 Publication for this product

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Thanks for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you dec...

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Thank you for your email. Purified recombinant human activin receptor IIB extracellular domain expressed in mouse NSO myeloma cells was used as the immunogen. Unfortunately we don't have any additional information regarding this antibody. If you have a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"