Anti-Activin Receptor Type IIA抗体(ab10595)

概述

  • 产品名称Anti-Activin Receptor Type IIA抗体
    参阅全部 Activin Receptor Type IIA 一抗
  • 描述
    山羊多克隆抗体to Activin Receptor Type IIA
  • 特异性The antibody exhibits less than 2 % cross-reactivity with recombinant human activin receptor I and recombinant human activin receptor IIB.
  • 经测试应用适用于: IHC-P, Indirect ELISA, IHC-Fr, WB, Neutralisingmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment (Human)from the Activin RIIA extracellular domain.

  • 常规说明


    Activin, a disulfide-linked homodimeric protein is secreted by Sertoli cells in the testis and granulosa cells in the ovary. In early studies, this peptide was thought to be an inhibin and not recognized as a unique compound. Activins and inhibins are members of the TGF-beta superfamily due to amino acid homology with respect to the conservation of 7 of the 9 cysteine residues common to all TGF-beta forms. Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. Five beta subunits have been cloned (mammalian betaA, betaB, betaC, betaE, and Xenopus betaD). The activin/inhibin nomenclature reflects the subunit composition of the proteins: activin A (betaA-betaA), activin B (betaB-betaB), activin AB (betaB-betaA), inhibin A (alpha-betaA), and inhibin B (alpha- betaB). Activins have a wide range of biological activities including mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis, and reproductive physiology. Activins are also involved in growth and differentiation of several tissues from different species. This protein also plays a key role in the production and regulation of hormones such as FSH, LH, GnRH, and ACTH. Activin influences erythropoiesis and the potentiation of erythroid colony formation, oxytocin secretion, paracrine, and autocrine regulation. Similar to other TGF-beta family members, activins exert their biological activities through the effects ot the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated type I and type receptors. Activin type I and type II receptors are distinguished by the level of sequence homology of their kinase domains and other structural and functional features. To date, seven type I and five type II activin receptors have been cloned from mammals, including activin receptor IA, activin receptor IIA, activin receptor IB, and activin receptor IIB. In addition, two splice variants of activin receptor IIA and five splice variants of activin receptor IIB have been reported. Type I activin receptors are highly conserved and do not bind directly to activin but will associate with the type II receptor-activin complex and initiate signal transduction. Type I activin receptors will also bind with the BMP-2/7-bound BMPR-II and form signaling complexes. Human, mouse, and bovine type IB activin receptors share greater than 98 % homology.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 5% Trehalose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • Primary antibody说明Activin, a disulfide-linked homodimeric protein is secreted by Sertoli cells in the testis and granulosa cells in the ovary. In early studies, this peptide was thought to be an inhibin and not recognized as a unique compound. Activins and inhibins are members of the TGF-beta superfamily due to amino acid homology with respect to the conservation of 7 of the 9 cysteine residues common to all TGF-beta forms. Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. Five beta subunits have been cloned (mammalian betaA, betaB, betaC, betaE, and Xenopus betaD). The activin/inhibin nomenclature reflects the subunit composition of the proteins: activin A (betaA-betaA), activin B (betaB-betaB), activin AB (betaB-betaA), inhibin A (alpha-betaA), and inhibin B (alpha- betaB). Activins have a wide range of biological activities including mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis, and reproductive physiology. Activins are also involved in growth and differentiation of several tissues from different species. This protein also plays a key role in the production and regulation of hormones such as FSH, LH, GnRH, and ACTH. Activin influences erythropoiesis and the potentiation of erythroid colony formation, oxytocin secretion, paracrine, and autocrine regulation. Similar to other TGF-beta family members, activins exert their biological activities through the effects ot the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated type I and type receptors. Activin type I and type II receptors are distinguished by the level of sequence homology of their kinase domains and other structural and functional features. To date, seven type I and five type II activin receptors have been cloned from mammals, including activin receptor IA, activin receptor IIA, activin receptor IB, and activin receptor IIB. In addition, two splice variants of activin receptor IIA and five splice variants of activin receptor IIB have been reported. Type I activin receptors are highly conserved and do not bind directly to activin but will associate with the type II receptor-activin complex and initiate signal transduction. Type I activin receptors will also bind with the BMP-2/7-bound BMPR-II and form signaling complexes. Human, mouse, and bovine type IB activin receptors share greater than 98 % homology.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 15 µg/ml.
Indirect ELISA Use a concentration of 0.5 - 1 µg/ml.
IHC-Fr Use at an assay dependent dilution. PubMed: 16835628
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 58 kDa. ~5 ng/lane under reducing and non-reducing conditions.
Neutralising Use a concentration of 1 - 3 µg/ml. The antibody will block 50% of the binding of 30 ng/ml recombinant human activin A to immobilized recombinant human activin receptor IIA/Fc chimera (100 µl of a 0.5 µg/ml solution coating each well) in a functional ELISA assay. ab10595 has not been optimized for cell/tissue culture conditions.

靶标

  • 功能On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin A, activin B and inhibin A.
  • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 protein kinase domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activin A receptor type IIA antibody
    • Activin receptor type 2A antibody
    • Activin receptor type IIA antibody
    • Activin receptor type-2A antibody
    • ACTR 2 antibody
    • ACTR IIA antibody
    • ACTR-IIA antibody
    • ACTR2 antibody
    • ACTRII antibody
    • ACTRIIA antibody
    • Acvr 2 antibody
    • Acvr 2A antibody
    • Acvr2 antibody
    • ACVR2A antibody
    • AVR2A_HUMAN antibody
    • OTTHUMP00000197918 antibody
    see all

Anti-Activin Receptor Type IIA antibody (ab10595)参考文献

This product has been referenced in:
  • Bauer J  et al. Activin and TGFß use diverging mitogenic signaling in advanced colon cancer. Mol Cancer 14:182 (2015). IHC-P ; Human . Read more (PubMed: 26497569) »
  • Liu XS  et al. Stroke induces gene profile changes associated with neurogenesis and angiogenesis in adult subventricular zone progenitor cells. J Cereb Blood Flow Metab 27:564-74 (2007). IHC-Fr ; Mouse . Read more (PubMed: 16835628) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (12% Gel)
Sample Mouse Tissue lysate - whole (Mouse Testis and Ovary. COV434 human cell line)
Specification Mouse Testis and Ovary. COV434 human cell line
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Dr. FRANCESCO ELIA MARINO

Verified customer

提交于 Dec 23 2014

Thank you for your call and for your patience.

Finally, I have some more information regarding the immunogen/epitopes of the the two antibodies you are interested in:


The immunogen used to raise ab10595 Anti-Activin Receptor Type...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. The following publication makes reference to the use of rat ovary as a positive control for IHC and RT-PCR analysis of Activin Receptor Type I...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"