RabMAb

Anti-Activin A Receptor Type IB抗体[EPR4815] (ab109300)

概述

  • 产品名称Anti-Activin A Receptor Type IB抗体[EPR4815]
    参阅全部 Activin A Receptor Type IB 一抗
  • 描述
    兔单克隆抗体[EPR4815] to Activin A Receptor Type IB
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    corresponding to Human Activin A Receptor Type IB.

  • 阳性对照
    • Human, mouse and rat brain tissue lysate; Human fetal kidney and liver tissue lysate; Human cervical carcinoma; Human kidney tissue; SH-SY5Y and U87-MG cell lysate.
  • 常规说明

    Alternative versions available:
    Anti-Activin A Receptor Type IB antibody (Biotin) [EPR4815] (ab204655)
    Anti-Activin A Receptor Type IB antibody (HRP) [EPR4815] (ab203725)

     

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab109300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 57 kDa).
IHC-P 1/1600. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

(Heat to 98°C, allow to cool for 10-20 minutes)

For Unpurified use at 1/500 - 1/1000.

ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Phosphorylates TDP2.
    • 组织特异性Expressed in many tissues, most strongly in kidney, pancreas, brain, lung, and liver.
    • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
      Contains 1 GS domain.
      Contains 1 protein kinase domain.
    • 翻译后修饰Autophosphorylated.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 形式There are 3 isoforms produced by alternative splicing. Isoform 1 also known as SKR2-1; isoform 2 as SKR2-2; isoform 3 as SKR2-3.
    • 别名
      • Activin A receptor type 1B antibody
      • Activin A receptor type II like kinase 4 antibody
      • Activin A type 1B receptor antibody
      • Activin A type IB receptor antibody
      • Activin receptor like kinase 4 antibody
      • Activin receptor type 1B antibody
      • Activin receptor type IB antibody
      • Activin receptor type-1B antibody
      • Activin receptor-like kinase 4 antibody
      • ACTR IB antibody
      • ACTR-IB antibody
      • ACTRIB antibody
      • ACV1B_HUMAN antibody
      • ACVR 1B antibody
      • Acvr1b antibody
      • ACVRLK 4 antibody
      • ACVRLK4 antibody
      • ALK 4 antibody
      • ALK-4 antibody
      • ALK4 antibody
      • Serine(threonine) protein kinase receptor R2 antibody
      • Serine/threonine-protein kinase receptor R2 antibody
      • SKR 2 antibody
      • SKR2 antibody
      see all

    Anti-Activin A Receptor Type IB antibody [EPR4815] 图像

    • Anti-Activin A Receptor Type IB antibody [EPR4815] (ab109300) at 1/1000 dilution (purified) + Human fetal kidney lysate at 15 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size : 57 kDa
      Observed band size : 52 kDa (why is the actual band size different from the predicted?)
      Blocking/Diluting buffer 5% NFDM/TBST
    • Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma sections labelling Activin A Receptor Type IB with purified ab109300 at dilution of 1/600. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was heat mediated using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

    • Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labelling Activin A Receptor Type IB with purified ab109300 at 1/250 (5.0 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000, 2 μg/mL) was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] (1/200, 2.5 μg/mL) - Microtubule Marker (Alexa Fluor® 594). DAPI (blue) was used as a nuclear counterstain.

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • All lanes : Anti-Activin A Receptor Type IB antibody [EPR4815] (ab109300) at 1/1000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 57 kDa
      Observed band size : 52 kDa (why is the actual band size different from the predicted?)
      Blocking/Diluting buffer 5% NFDM/TBST
    • All lanes : Anti-Activin A Receptor Type IB antibody [EPR4815] (ab109300) at 1/1000 dilution (unpurified)

      Lane 1 : Human brain tissue lysate
      Lane 2 : Human fetal kidney
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : U87-MG cell lysate
      Lane 5 : Human fetal liver

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 57 kDa
      Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of human kidney tissue sections labelling Activin A receptor type IB with unpurified ab109300 at 1/500 dilution. 

    • Immunocytochemistry/ Immunofluorescence analysis of 2% paraformaldehyde fixed (20 mins) SH-SY5Y cells labelling Activin A Receptor Type IB with unpurified ab109300 at a dilution of 1/100. Alexa Fluor488 goat anti-rabbit IgG was used as the secondary antibody. 

    Anti-Activin A Receptor Type IB antibody [EPR4815] (ab109300)参考文献

    This product has been referenced in:
    • Kaiser O  et al. TGF-beta superfamily member activin A acts with BDNF and erythropoietin to improve survival of spiral ganglion neurons in vitro. Neuropharmacology 75C:416-425 (2013). Read more (PubMed: 23973291) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (eye)
    Specification eye
    Fixative Formaldehyde
    Permeabilization No
    Blocking step 0.5% BSA + 5% Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 12 2013

    Merci de nous avoir contactés.


    La concentration de ab109300 de notre lot en stock (GR56935-5) est xxxxxxxxxx.

    J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nou...

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    I am very pleased to hear you would like to accept our offer and test ab109300 in IHC-FoFr (pfa fixed, frozen). This code will give you $*** off your next order before the expiration date. To redeem this offer, please submit an Abreview for IHC-Fo...

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    Vielen Dank für Ihre Anfrage.

    Ich habe gute Neuigkeiten: ich werde das Datenblatt des ab109300 heute oder morgen noch mit dem neuen ICC Bild auf den neusten Stand bringen. Desweiteren habe ich dies an ebenfalls als weitere Email an sie ge...

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    Vielen Dank für Ihren Anruf.

    Ich habe mich in die Materie gearbeitet und herausgefunden, dass dieses sich Protein zwischen der Zellmembran und dem Zytoplasma hin und her bewegt.

    Unser Labor hat zwar im Moment kein Bild vom ab10...

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