Anti-Activin A Receptor Type IB抗体(ab64813)

概述

  • 产品名称Anti-Activin A Receptor Type IB抗体
    参阅全部 Activin A Receptor Type IB 一抗
  • 描述
    兔多克隆抗体to Activin A Receptor Type IB
  • 经测试应用适用于: WB, ELISA, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide derived from an internal region of human Activin A Receptor Type IB

  • 阳性对照
    • Hela cells or 293 cell lines. Heart.

性能

应用

Our Abpromise guarantee covers the use of ab64813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Phosphorylates TDP2.
  • 组织特异性Expressed in many tissues, most strongly in kidney, pancreas, brain, lung, and liver.
  • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 GS domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 形式There are 3 isoforms produced by alternative splicing. Isoform 1 also known as SKR2-1; isoform 2 as SKR2-2; isoform 3 as SKR2-3.
  • 别名
    • Activin A receptor type 1B antibody
    • Activin A receptor type II like kinase 4 antibody
    • Activin A type 1B receptor antibody
    • Activin A type IB receptor antibody
    • Activin receptor like kinase 4 antibody
    • Activin receptor type 1B antibody
    • Activin receptor type IB antibody
    • Activin receptor type-1B antibody
    • Activin receptor-like kinase 4 antibody
    • ACTR IB antibody
    • ACTR-IB antibody
    • ACTRIB antibody
    • ACV1B_HUMAN antibody
    • ACVR 1B antibody
    • Acvr1b antibody
    • ACVRLK 4 antibody
    • ACVRLK4 antibody
    • ALK 4 antibody
    • ALK-4 antibody
    • ALK4 antibody
    • Serine(threonine) protein kinase receptor R2 antibody
    • Serine/threonine-protein kinase receptor R2 antibody
    • SKR 2 antibody
    • SKR2 antibody
    see all

Anti-Activin A Receptor Type IB antibody 图像

  • Immunofluorescence analysis of HeLa cells using ab64813 at a 1:500 dilution.
    Left image untreated.
    Right image treated with peptide.
  • All lanes : Anti-Activin A Receptor Type IB antibody (ab64813) at 1/500 dilution

    Lane 1 : Extracts from 293 cells
    Lane 2 : Extracts from mouse heart
    Lane 3 : Extracts from mouse heart cells immunizing peptide at 5 µg

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 57 kDa
    Observed band size : 57 kDa
  • ab64813 staining Activin A Receptor Type IB in human lymph node tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/250 and incubated with the sample for 12 hours at 4°C. An HRP-conjugated goat anti-rabbit polyclonal was used as the secondary antibody, diluted 1/200.

    See Abreview

Anti-Activin A Receptor Type IB antibody (ab64813)参考文献

This product has been referenced in:
  • Peng Y  et al. Critical roles of miRNA-mediated regulation of TGFß signalling during mouse cardiogenesis. Cardiovasc Res 103:258-67 (2014). Mouse . Read more (PubMed: 24835278) »

See 1 Publication for this product

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Ich habe mich in die Materie gearbeitet und herausgefunden, dass dieses sich Protein zwischen der Zellmembran und dem Zytoplasma hin und her bewegt.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lymph node)
Specification Lymph node
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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提交于 Jun 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"