Anti-Activin A Receptor Type IB抗体(ab10594)

概述

  • 产品名称Anti-Activin A Receptor Type IB抗体
    参阅全部 Activin A Receptor Type IB 一抗
  • 描述
    山羊多克隆抗体to Activin A Receptor Type IB
  • 特异性Based on immunoblotting, this antibody exhibits approximately 2 % cross-reactivity with recombinant human activin receptor IIA and recombinant human activin receptor IA and less than 1 % cross-reactivity with recombinant human activin receptor IIA and recombinant human activin receptor IIB.
  • 经测试应用适用于: IHC-P, IHC-FoFr, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    purified recombinant human activin receptor IB extracellular domain expressed in mouse NSO cells.

  • 阳性对照
    • human breast carcinoma
  • 常规说明


    Activin, a disulfide-linked homodimeric protein is secreted by Sertoli cells in the testis and granulosa cells in the ovary. In early studies, this peptide was thought to be an inhibin and not recognized as a unique compound. Activins and inhibins are members of the TGF-beta superfamily due to amino acid homology with respect to the conservation of 7 of the 9 cysteine residues common to all TGF-beta forms. Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. Five beta subunits have been cloned (mammalian betaA, betaB, betaC, betaE, and Xenopus betaD). The activin/inhibin nomenclature reflects the subunit composition of the proteins: activin A (betaA-betaA), activin B (betaB-betaB), activin AB (betaB-betaA), inhibin A (alpha-betaA), and inhibin B (alpha- betaB). Activins have a wide range of biological activities including mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis, and reproductive physiology. Activins are also involved in growth and differentiation of several tissues from different species. This protein also plays a key role in the production and regulation of hormones such as FSH, LH, GnRH, and ACTH. Activin influences erythropoiesis and the potentiation of erythroid colony formation, oxytocin secretion, paracrine, and autocrine regulation. Similar to other TGF-beta family members, activins exert their biological activities through the effects ot the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated type I and type receptors. Activin type I and type II receptors are distinguished by the level of sequence homology of their kinase domains and other structural and functional features. To date, seven type I and five type II activin receptors have been cloned from mammals, including activin receptor IA, activin receptor IIA, activin receptor IB, and activin receptor IIB. In addition, two splice variants of activin receptor IIA and five splice variants of activin receptor IIB have been reported. Type I activin receptors are highly conserved and do not bind directly to activin but will associate with the type II receptor-activin complex and initiate signal transduction. Type I activin receptors will also bind with the BMP-2/7-bound BMPR-II and form signaling complexes. Human, mouse, and bovine type IB activin receptors share greater than 98 % homology.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • Primary antibody说明Activin, a disulfide-linked homodimeric protein is secreted by Sertoli cells in the testis and granulosa cells in the ovary. In early studies, this peptide was thought to be an inhibin and not recognized as a unique compound. Activins and inhibins are members of the TGF-beta superfamily due to amino acid homology with respect to the conservation of 7 of the 9 cysteine residues common to all TGF-beta forms. Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. Five beta subunits have been cloned (mammalian betaA, betaB, betaC, betaE, and Xenopus betaD). The activin/inhibin nomenclature reflects the subunit composition of the proteins: activin A (betaA-betaA), activin B (betaB-betaB), activin AB (betaB-betaA), inhibin A (alpha-betaA), and inhibin B (alpha- betaB). Activins have a wide range of biological activities including mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis, and reproductive physiology. Activins are also involved in growth and differentiation of several tissues from different species. This protein also plays a key role in the production and regulation of hormones such as FSH, LH, GnRH, and ACTH. Activin influences erythropoiesis and the potentiation of erythroid colony formation, oxytocin secretion, paracrine, and autocrine regulation. Similar to other TGF-beta family members, activins exert their biological activities through the effects ot the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated type I and type receptors. Activin type I and type II receptors are distinguished by the level of sequence homology of their kinase domains and other structural and functional features. To date, seven type I and five type II activin receptors have been cloned from mammals, including activin receptor IA, activin receptor IIA, activin receptor IB, and activin receptor IIB. In addition, two splice variants of activin receptor IIA and five splice variants of activin receptor IIB have been reported. Type I activin receptors are highly conserved and do not bind directly to activin but will associate with the type II receptor-activin complex and initiate signal transduction. Type I activin receptors will also bind with the BMP-2/7-bound BMPR-II and form signaling complexes. Human, mouse, and bovine type IB activin receptors share greater than 98 % homology.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
IHC-FoFr Use a concentration of 5 - 15 µg/ml.

Use ABC amplification.

WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 57 kDa.

Detects human activin receptor IB at approximately 5 ng/lane under reducing and non-reducing conditions.

ELISA Use a concentration of 0.5 - 1 µg/ml.

靶标

  • 功能On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Phosphorylates TDP2.
  • 组织特异性Expressed in many tissues, most strongly in kidney, pancreas, brain, lung, and liver.
  • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 GS domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 形式There are 3 isoforms produced by alternative splicing. Isoform 1 also known as SKR2-1; isoform 2 as SKR2-2; isoform 3 as SKR2-3.
  • 别名
    • Activin A receptor type 1B antibody
    • Activin A receptor type II like kinase 4 antibody
    • Activin A type 1B receptor antibody
    • Activin A type IB receptor antibody
    • Activin receptor like kinase 4 antibody
    • Activin receptor type 1B antibody
    • Activin receptor type IB antibody
    • Activin receptor type-1B antibody
    • Activin receptor-like kinase 4 antibody
    • ACTR IB antibody
    • ACTR-IB antibody
    • ACTRIB antibody
    • ACV1B_HUMAN antibody
    • ACVR 1B antibody
    • Acvr1b antibody
    • ACVRLK 4 antibody
    • ACVRLK4 antibody
    • ALK 4 antibody
    • ALK-4 antibody
    • ALK4 antibody
    • Serine(threonine) protein kinase receptor R2 antibody
    • Serine/threonine-protein kinase receptor R2 antibody
    • SKR 2 antibody
    • SKR2 antibody
    see all

Anti-Activin A Receptor Type IB antibody 图像

  • Human breast carcinoma tissue stained with ab10594 (5ug/ml). See recommended protocol for further details.

Anti-Activin A Receptor Type IB antibody (ab10594)参考文献

ab10594 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Breast)
Specification Breast
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C
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提交于 May 12 2009

We have just received a detailed protocol from the source of ab10594 who has tested the antibody in IHC on breast carcinoma tissue. I enclose the protocol and hope this will help you. If you are still not satisfied with the product please let me kno...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"