重组人IGF1蛋白(Active) (ab155614)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: Fc tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies, ELISA
Achieve higher consistency and quality standards with a premium grade bioactive protein
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
描述
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产品名称
重组人IGF1蛋白(Active)
参阅全部 IGF1 蛋白酶 -
生物活性
Measured by its binding ability in a functional ELISA. Immobilized Human IGF-I R, His Tag (ab155622) at 5 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag (ab155614) with a linear range of 0.039-0.313 μg/mL.
Measured by its binding ability in a functional ELISA. Immobilized Recombinant human IGF1 protein (Active) (ab155614) at 5 μg/mL (100 μL/well) can bind Recombinant Human IGFBP3 protein (ab155611) with a linear range of 0.031-0.5 μg/mL.
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纯度
> 98 % SDS-PAGE.
Lyophilized from 0.22µm filtered solution -
内毒素水平
< 1.000 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
GPETLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFR SCDLRRLEMYCAPLKPAKSA -
预测分子量
35 kDa including tags -
氨基酸
49 to 118 -
标签
Fc tag N-Terminus
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技术指标
Our Abpromise guarantee covers the use of ab155614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
ELISA
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 0.75% Glycine, 0.605% Tris
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
常规信息
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别名
- IBP1
- IGF I
- IGF IA
see all -
功能
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
疾病相关
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
序列相似性
Belongs to the insulin family. -
细胞定位
Secreted. - Information by UniProt
图片
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Immobilized Recombinant human IGF1 protein (Active) (ab155614) at 5 μg/mL (100 μL/well) can bind Recombinant Human IGFBP3 protein (ab155611) with a linear range of 0.031-0.5 μg/mL.
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Immobilized Human IGF-I R, His Tag (ab155622) at 5 μg/mL (100 μL/well) can bind Recombinant human IGF1 protein (Active) (ab155614) with a linear range of 0.039-0.313 μg/mL.
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SDS-PAGE of reduced ab155614 stained overnight with Coomassie Blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab155614 被引用在 1 文献中.
- Kasprzycka P et al. The factors present in regenerating muscles impact bone marrow-derived mesenchymal stromal/stem cell fusion with myoblasts. Stem Cell Res Ther 10:343 (2019). PubMed: 31753006