Anti-active Caspase 7抗体(ab2323)


  • 产品名称
    Anti-active Caspase 7抗体
  • 描述
    兔多克隆抗体to active Caspase 7
  • 宿主
  • 特异性
    This antibody recognizes only the large subunit of cleaved caspase 7. The antibody does not recognize full length caspase 7 or any other caspase.
  • 经测试应用
    适用于: IHC-Fr, IP, WB, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human active Caspase 7. Synthetic peptide surrounding amino acid 195 of Human Caspase-7.


  • 形式
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Thimerosal (merthiolate)
    Constituents: PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • Primary antibody说明
    Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 7 has been identified as a major contributor to the execution of apoptosis. Caspase 7 is cleaved by many enzymes, including caspase 3, 6, 8, 9 and granzyme B. Active caspase 7 consists of a heterotetramer of two large and two small subunits. As an effector caspase, caspase 7 cleaves many of the same substrates as caspase 3, including poly (ADP-ribose) polymerase (PARP). The active caspase 7 antibody provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
  • 克隆
  • 同种型
  • 研究领域


Our Abpromise guarantee covers the use of ab2323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use a concentration of 10 - 20 µg/ml.
IP Use a concentration of 10 - 20 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.



  • 相关性
    Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.
  • 细胞定位
  • 数据库链接
  • 别名
    • Apoptotic protease Mch3 antibody
    • CASP 7 antibody
    • Caspase 7 subunit p11 antibody
    • Caspase 7 subunit p20 antibody
    • CMH1 antibody
    • ICE LAP3 antibody
    • ICE like apoptotic protease 3 antibody
    see all


  • All lanes : Anti-active Caspase 7 antibody (ab2323) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Mouse hepatocyte cell lysate

    Lysates/proteins at 25 µg per lane.

    All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 20 kDa (why is the actual band size different from the predicted?)

    Exposure time: 2 minutes

    See Abreview

  • Anti-active Caspase 7 antibody (ab2323) at 1 µg/ml
    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 20 kDa (why is the actual band size different from the predicted?)

    Jurkat cells induced with 2 mM of Camptothecin for 6 hours


This product has been referenced in:
  • Ge J  et al. Knockdown of Rab21 inhibits proliferation and induces apoptosis in human glioma cells. Cell Mol Biol Lett 22:30 (2017). WB ; Human . Read more (PubMed: 29270202) »
  • Wang-Johanning F  et al. Immunotherapeutic potential of anti-human endogenous retrovirus-k envelope protein antibodies in targeting breast tumors. J Natl Cancer Inst 104:189-210 (2012). WB ; Human . Read more (PubMed: 22247020) »

See all 4 Publications for this product


Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Mouse Cell lysate - whole cell (LIVER)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

提交于 Nov 17 2014

I'm sorry to hear you are having a problem with ab2323 I would like to suggest the following modifications to your protocol: block for 1 hour at room temperature in 5% milk, incubate the primary at 1 ug/mL in blocking buffer overnight at 4 degrees,...

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Thank you for your enquiry. According to the datasheet, this antibody has not been tested on formalin-fixed tissue sections (only on frozen sections). Should you decide to go ahead and test this antibody, please let us know how you get on, and in...

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I can tell you that we did not soucre this antibody from cell signalling but our suppliers names are commercially sensitive information. The antibody should be used at 10-20ug/ml in frozen sections and antigen retrieval is not required although it may ...

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