Anti-active Caspase-3抗体(ab49822)


  • 产品名称Anti-active Caspase-3抗体
    参阅全部 active Caspase-3 一抗
  • 描述
    兔多克隆抗体to active Caspase-3
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Dog, Human, Pig
  • 免疫原

    Synthetic peptide corresponding to Human active Caspase-3 aa 167-175 conjugated to Keyhole Limpet Haemocyanin (KLH).


    Database link: P42574

  • 阳性对照
    • Human epitheloid carcinoma HeLa cell line, treated with staurosporine and Human recombinant caspase-3, active.



Our Abpromise guarantee covers the use of ab49822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500.
ICC/IF 1/1000.


  • 功能Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
  • 组织特异性Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • 序列相似性Belongs to the peptidase C14A family.
  • 翻译后修饰Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apopain antibody
    • CASP 3 antibody
    • CASP-3 antibody
    • CASP3 antibody
    • CASP3_HUMAN antibody
    • Caspase 3 antibody
    • Caspase-3 subunit p12 antibody
    • CPP 32 antibody
    • CPP-32 antibody
    • CPP32B antibody
    • Cysteine protease CPP32 antibody
    • PARP cleavage protease antibody
    • Protein Yama antibody
    • SCA-1 antibody
    • SCA1 antibody
    • SREBP cleavage activity 1 antibody
    • Yama antibody
    see all

Anti-active Caspase-3 antibody 图像

  • Anti-active Caspase-3 antibody (ab49822) at 1/1000 dilution

    Goat Anti-Rabbit IgG, Peroxidase conjugate
    Developed using the ECL technique

    Observed band size : 17 kDa (why is the actual band size different from the predicted?)

Anti-active Caspase-3 antibody (ab49822)参考文献

This product has been referenced in:
  • Huang G  et al. Notoginsenoside R1 attenuates glucose-induced podocyte injury via the inhibition of apoptosis and the activation of autophagy through the PI3K/Akt/mTOR signaling pathway. Int J Mol Med 39:559-568 (2017). Human . Read more (PubMed: 28112381) »
  • Howie RN  et al. Effects of In Utero Thyroxine Exposure on Murine Cranial Suture Growth. PLoS One 11:e0167805 (2016). WB ; Mouse . Read more (PubMed: 27959899) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Sheep Tissue sections (Thymus)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Commercial Retrieval Solution
Permeabilization No
Specification Thymus
Blocking step (agent) for 10 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative Formaldehyde

Abcam user community

Verified customer

提交于 Sep 15 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Ferret Tissue sections (Small Intestines)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Commercial Retrieval Solution
Permeabilization No
Specification Small Intestines
Fixative Formaldehyde

Fabian Lean

Verified customer

提交于 Jul 19 2016

This antibody is specific to the active form of caspase 3.