Anti-active Caspase-3抗体(ab2302)

概述

• 产品名称Anti-active Caspase-3抗体
  参阅全部 active Caspase-3 一抗
• 描述
  兔多克隆抗体to active Caspase-3
• 特异性The antibody preferentially recognizes the p17 fragment of the active Caspase-3. Under the conditions we tested, the antibody did not detect the precursor form. Under other conditions (such as those used in PMC2206181), this antibody has been found to detect both the pro and active forms of Caspase-3. Although some customers have used this antibody successfully in mouse and rat, we do not batch test in these species and so cannot guarantee that it will work in them.
• 经测试应用适用于: IHC-FoFr, IHC-P, IHC-Fr, WBmore details
• 种属反应性
  与反应: Rabbit, Human, Quail
  预测可用于: Mouse, Rat
• 免疫原

  Synthetic peptide corresponding to Human active Caspase-3. Synthetic peptide mapping to the N-terminus adjacent to the cleavage site of human caspase-3
  Database link: P42574
  (Peptide available as ab38283)

• 阳性对照
  • Camptothecin (2 µM) treated Jurkat cells. If no signal is observed a time course may be required to identify maximal caspase activity.
• 常规说明

   

  Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase 3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). This affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
  Constituents: PBS, 30% Glycerol, 0.5% BSA
• Concentration information loading...
• 纯度Immunogen affinity purified
• Primary antibody说明Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase 3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). This affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
• 克隆多克隆
• 同种型IgG
• 研究领域
  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Other

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Caspases etc
  • Caspases

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Other

  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Caspases

  • Cancer
  • Cell Death
  • Apoptosis
  • Apoptotic Markers
  • Caspases

相关产品

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
• Immunizing Peptide (Blocking)
  • Human Caspase-3 peptide (ab38283)

应用

靶标

• 功能Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
  -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
• 组织特异性Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
• 序列相似性Belongs to the peptidase C14A family.
• 翻译后修饰Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
  S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
• 细胞定位Cytoplasm.

• Target information above from: UniProt accession P42574 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 数据库链接
  • Entrez Gene: 836 Human
  • Entrez Gene: 12367 Mouse
  • Entrez Gene: 25402 Rat
  • Omim: 600636 Human
  • SwissProt: P42574 Human
  • SwissProt: P70677 Mouse
  • SwissProt: Q8MJC3 Rabbit
  • SwissProt: P55213 Rat

  • Unigene: 141125 Human
  • Unigene: 34405 Mouse
  • Unigene: 10562 Rat

  see all
• 别名
  • Apopain antibody
  • CASP 3 antibody
  • CASP-3 antibody

  • CASP3 antibody
  • CASP3_HUMAN antibody
  • Caspase 3 antibody
  • Caspase-3 subunit p12 antibody
  • CPP 32 antibody
  • CPP-32 antibody
  • CPP32B antibody
  • Cysteine protease CPP32 antibody
  • PARP cleavage protease antibody
  • Protein Yama antibody
  • SCA-1 antibody
  • SCA1 antibody
  • SREBP cleavage activity 1 antibody
  • Yama antibody

  see all

实验方案

• Recommended protocol for WB with ab2302