Application
Western blot
Sample
Mouse Cell lysate - whole cell (Brain Tissue and Coronal Suture Cell whole cell ly)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
8 µg
Treatment
10 µM H2O2 for 6 hrs
Specification
Brain Tissue and Coronal Suture Cell whole cell ly
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk/TBS-T
Dilution
1/250
Detection
Detection method
Clarity ECL
Exposure
20 minute(s) and 0 second(s)
Positive control
Mouse coronal suture cells whole cell lysate + H202 treatment for 6 hours at 4, 8 and 12 µg/well
Additional data
Additional Notes
1. Mouse brain tissue whole cell lysate 1: 8 µg/well
2. Mouse brain tissue whole cell lysate 2: 8 µg/well
3. Mouse brain tissue whole cell lysate 3: 8 µg/well
4. Mouse coronal suture cells whole cell lysate + H202 treatment: 4 µg/well positive control
5. Mouse coronal suture cells whole cell lysate + H202 treatment: 8 µg/well positive control
6. Mouse coronal suture cells whole cell lysate + H202 treatment: 12 µg/well positive control
Abcam response
Thank you for taking part in our Abtrial testing program for mouse reactivity. Your results suggest that this product may not cross react with this species. We welcome additional feedback.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jun 03 2016