概述

  • 产品名称Anti-activated Notch1抗体
    参阅全部 activated Notch1 一抗
  • 描述
    兔多克隆抗体to activated Notch1
  • 特异性ab52301 detects endogenous levels of fragment of activated Notch 1 resulting from cleavage adjacent to Val1744.
  • 经测试应用适用于: ICC/IF, IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human activated Notch1 aa 1720-1770.
    Sequence:

    A VQSETVEPPP PAQLHFMYVA AAAFVLLFFV GCGVLLSRKR RRQHGQLWFP


    Database link: P46531

  • 阳性对照
    • Extracts from NIH 3T3 cells, treated with etoposide (25µM, 1hour).
  • 常规说明

    ab52301 is cleaved at Val 1744

性能

应用

Our Abpromise guarantee covers the use of ab52301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 20680961
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 272 kDa).
ELISA 1/10000.

靶标

  • 功能Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May enhance HIF1A function by sequestering HIF1AN away from HIF1A.
  • 组织特异性In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
  • 疾病相关Defects in NOTCH1 are a cause of aortic valve disease 1 (AOVD1) [MIM:109730]. A common defect in the aortic valve in which two rather than three leaflets are present. It is often associated with aortic valve calcification and insufficiency. In extreme cases, the blood flow may be so restricted that the left ventricle fails to grow, resulting in hypoplastic left heart syndrome.
  • 序列相似性Belongs to the NOTCH family.
    Contains 5 ANK repeats.
    Contains 36 EGF-like domains.
    Contains 3 LNR (Lin/Notch) repeats.
  • 翻译后修饰Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). Following endocytosis, this fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
    Phosphorylated.
    O-glycosylated on the EGF-like domains. Contains both O-linked fucose and O-linked glucose.
    Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH. Monoubiquitination at Lys-1759 is required for activation by gamma-secretase cleavage, it promotes interaction with AAK1, which stabilizes it. Deubiquitination by EIF3F is necessary for nuclear import of activated Notch.
    Hydroxylated at Asn-1955 by HIF1AN. Hydroxylated at Asn-2022 by HIF1AN (By similarity). Hydroxylation reduces affinity for HI1AN and may thus indirectly modulate negative regulation of NICD.
  • 细胞定位Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hN1 antibody
    • Neurogenic locus Notch homolog protein 1 antibody
    • NICD antibody
    • NOTC1_HUMAN antibody
    • Notch 1 antibody
    • Notch 1 intracellular domain antibody
    • Notch homolog 1 translocation associated (Drosophila) antibody
    • notch1 antibody
    • TAN1 antibody
    • Translocation-associated notch protein TAN-1 antibody
    see all

Anti-activated Notch1 antibody 图像

  • Immunofluorescence analysis of frozen tissue sections (10µm) of intestine from sham operated rats (control group) or rats after Ischemic-Reperfusion (I/R) injury labeling Notch1 with ab52301. Tissue sections were frozen after being fixed with 4% formaldehyde, then incubated in 3% H2Ofor 30 minutes and then blocked with 5% BSA for 30 mins at room temperature. The sections were incubated overnight with ab52301 (PBS + 3% BSA). An appropriate  secondary antibody was then added. DAPI (in blue) was used to stain the nucleus.

  • All lanes : Anti-activated Notch1 antibody (ab52301) at 1/500 dilution

    Lane 1 : NIH 3T3 cell extract treated with etoposide (25µM, 1 hour)
    Lane 2 : NIH 3T3 cell extract treated with etoposide (25µM, 1 hour) with immunising peptide


    Predicted band size : 272 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)

Anti-activated Notch1 antibody (ab52301)参考文献

This product has been referenced in:
  • Chang BS  et al. Collagen complexes increase the efficiency of iPS cells generated using fibroblasts from adult mice. J Reprod Dev 61:145-53 (2015). WB ; Mouse . Read more (PubMed: 25740096) »
  • Wu J  et al. Downregulation of microRNA-30 facilitates podocyte injury and is prevented by glucocorticoids. J Am Soc Nephrol 25:92-104 (2014). WB ; Mouse . Read more (PubMed: 24029422) »

See all 7 Publications for this product

Product Wall



Ab52301 does detect the activated, intracellular domain of Notch 1 that forms after cleavage at Valine 1744 (murine) or Valine 1754 (human).

Yes, the protease inhibitors may help, and to get bands in all lanes at 120 kDa, it may simply be necessary to develop the blot more: load more sample, use more antibody (for example 1/250), and incubate overnight at 4C in a solution of 1% BSA instead ...

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Thank you for bringing this to our attention and for sending your protocol. I think your protocol is fine. I am a little concerned about the quality of the samples. From what you have described, there may be some degradation which would account for the...

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Thank you for contacting us. We have a number of anti-Notch1 antibodies in our catalogue, however, none are specifically raised to detect the transmembrane domain. The transmembrane domain of human Notch1 is composed of residues 1736-1756 (SwissProt: P...

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Thank you for contacting Abcam. I have talked to the lab about whether ab52301 recognizes other bands. They have told me that the antibody can detect endogenous levels of fragment of activated Notch 1 resulting from cleavage adjacent to Val1754. The ba...

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