Anti-Actin Regulatory蛋白CAPG/MCP抗体(ab90873)
Key features and details
- Rabbit polyclonal to Actin Regulatory Protein CAPG/MCP
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Actin Regulatory蛋白CAPG/MCP抗体
参阅全部 Actin Regulatory Protein CAPG/MCP 一抗 -
描述
兔多克隆抗体to Actin Regulatory蛋白CAPG/MCP -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow -
免疫原
Synthetic peptide corresponding to Human Actin Regulatory Protein CAPG/MCP aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab101225) -
阳性对照
- Recombinant Human Actin Regulatory Protein CAPG/MCP (ab95385) can be used as a positive control in WB. This antibody gave a positive signal in the following whole cell lysates: HL60; U937; THP1; RAW 264.7.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab90873于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
靶标
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功能
Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA. -
组织特异性
Macrophages and macrophage-like cells. -
序列相似性
Belongs to the villin/gelsolin family.
Contains 3 gelsolin-like repeats. -
翻译后修饰
The N-terminus is blocked. -
细胞定位
Cytoplasm. Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 353121 Cow
- Entrez Gene: 822 Human
- Entrez Gene: 12332 Mouse
- Entrez Gene: 297339 Rat
- Omim: 153615 Human
- SwissProt: Q865V6 Cow
- SwissProt: P40121 Human
- SwissProt: P24452 Mouse
see all -
别名
- Actin capping protein GCAP39 antibody
- Actin regulatory protein CAP G antibody
- Actin regulatory protein CAP-G antibody
see all
图片
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All lanes : Anti-Actin Regulatory Protein CAPG/MCP antibody (ab90873) at 1 µg/ml
Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lane 2 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate
Lane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lane 4 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 10 µg/ml per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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