Anti-Actin抗体(Biotin) (ab113279)

概述

  • 产品名称Anti-Actin抗体(Biotin)
    参阅全部 Actin 一抗
  • 描述
    兔多克隆抗体to Actin (Biotin)
  • 偶联物Biotin
  • 特异性The epitope recognized by ab113279 maps to the N-terminus of Human Beta-actin using the numbering given in Swiss-Prot entry P60709 (GeneID 60). The N-terminus of Beta-Actin is highly conserved with Gamma-Actin and preliminary indications are that ab113279 also recognizes Gamma- Actin
  • 经测试应用适用于: WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Snake, a wide range of other species, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis , Medaka fish
  • 免疫原

    Synthetic peptide corresponding to Human Actin (N terminal).

  • 阳性对照
    • HeLa whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab113279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/15000. Predicted molecular weight: 42 kDa.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
    • 疾病相关Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
      Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
      Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
    • 序列相似性Belongs to the actin family.
    • 细胞定位Cytoplasm > cytoskeleton.
    • Information by UniProt
    • 数据库链接
    • 别名
      • a actin antibody
      • ACTA antibody
      • ACTA1 antibody
      • Actin alpha skeletal muscle antibody
      • Actin antibody
      • actin, alpha 1, skeletal muscle 1 antibody
      • actin, alpha 1, skeletal muscle antibody
      • Actin, alpha skeletal muscle antibody
      • actina antibody
      • actine antibody
      • ACTS_HUMAN antibody
      • aktin antibody
      • Alpha Actin 1 antibody
      • Alpha skeletal muscle Actin antibody
      • alpha skeletal muscle antibody
      • alpha-actin antibody
      • Alpha-actin-1 antibody
      • ASMA antibody
      • CFTD antibody
      • CFTD1 antibody
      • CFTDM antibody
      • MPFD antibody
      • NEM1 antibody
      • NEM2 antibody
      • NEM3 antibody
      • nemaline myopathy type 3 antibody
      see all

    Anti-Actin antibody (Biotin) 图像

    • All lanes : Anti-Actin antibody (Biotin) (ab113279) at 0.07 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 42 kDa


      Exposure time : 30 seconds

    Anti-Actin antibody (Biotin) (ab113279)参考文献

    ab113279 has not yet been referenced specifically in any publications.

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