Anti-Actin抗体[ACTN05] (Biotin) (ab79667)


  • 产品名称Anti-Actin抗体[ACTN05] (Biotin)
    参阅全部 Actin 一抗
  • 描述
    小鼠单克隆抗体[ACTN05] to Actin (Biotin)
  • 偶联物Biotin
  • 特异性Reacts with all six known vertebrate isoforms of actin (MW~42kDa). Also reacts with two cytoplasmic actins (beta, gamma) which are highly homologous to one another but differ from the muscle actins at about 25 amino acid residues.
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Pig, Dictyostelium discoideum, Physarum polycephalum
  • 免疫原

    Chicken gizzard Actin

  • 阳性对照
    • HeLa cells. Skeletal muscle.
  • 常规说明

    Highly recommended for monitoring total protein load on Western blots.



Our Abpromise guarantee covers the use of ab79667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

Use for 2hrs at RT.

IP Use at 2 µg/mg of lysate.

Verified on denatured Actin. Use protein G.

IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use for 30 min with LV’s UltraVision. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.


  • 功能Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • 疾病相关Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • 序列相似性Belongs to the actin family.
  • 细胞定位Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • a actin antibody
    • ACTA antibody
    • ACTA1 antibody
    • Actin alpha skeletal muscle antibody
    • Actin antibody
    • actin, alpha 1, skeletal muscle 1 antibody
    • actin, alpha 1, skeletal muscle antibody
    • Actin, alpha skeletal muscle antibody
    • actina antibody
    • actine antibody
    • ACTS_HUMAN antibody
    • aktin antibody
    • Alpha Actin 1 antibody
    • Alpha skeletal muscle Actin antibody
    • alpha skeletal muscle antibody
    • alpha-actin antibody
    • Alpha-actin-1 antibody
    • ASMA antibody
    • CFTD antibody
    • CFTD1 antibody
    • CFTDM antibody
    • MPFD antibody
    • NEM1 antibody
    • NEM2 antibody
    • NEM3 antibody
    • nemaline myopathy type 3 antibody
    see all

Anti-Actin antibody [ACTN05] (Biotin) (ab79667)参考文献

ab79667 has not yet been referenced specifically in any publications.

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I just received an update form lab regarding the epitope for ab79667 and ab113279. Unfortunately the epitope has not been determined so the information is not available. I am sorry we will not be able to suggest th...

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I can confirm that I am still waiting for information from lab. I will send you the epitoope details of ab79667 as soon as I receive them.

The other possible combination of ab8227 in sandwich ELISA would b...

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Thank you for contacting Abcam regarding finding biotin labeled primary antibodies and control tissues.

After review of our catalog, my first recommendation is anti-Collagen VI, ab119300. This product is guaranteed in IHC-P and the images on...

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