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Chicken gizzard Actin
Highly recommended for monitoring total protein load on Western blots.
Our Abpromise guarantee covers the use of ab79667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
Use for 2hrs at RT.
|IP||Use at 2 µg/mg of lysate.
Verified on denatured Actin. Use protein G.
|IHC-P||1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use for 30 min with LV’s UltraVision. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
ab79667 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"