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Synthetic peptide within Human Actin aa 365-375 (C terminal). The exact sequence is proprietary.
This product was changed from ascites to tissue culture supernatant on 1st November 2016. The following lot is from ascites and is still in stock as of 1st November 2016 - GR158107. Lot numbers higher than GR158107 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Our Abpromise guarantee covers the use of ab11003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent concentration.
Use at an assay dependent concentration
|WB||Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa.
|ELISA||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.
1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts.
ab11003 staining Actin in Human SW480 cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed and then blocked using 10% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/50 for 2 hours at 25°C. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 568 (red) used at a 1/100 dilution.
Immunohistochemical analysis of frozen Human tongue tissue, using ab11003.
ab11003 staining Human fibroblast cells by ELISA (Sandwich-capture). Cells were blocked in 1% BSA for 1 hour at 25°C. The primary antibody ab11003 (Capture antibody) was diluted 1/3000 and incubated with sample for 12 hours at 4°C. ab5694 was used as secondary and a HRP conjugated goat polyclonal to mouse, diluted 1/1000 was used as detection antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"