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Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab219733 as a replacement.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Our Abpromise guarantee covers the use of ab11003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent concentration.
Use at an assay dependent concentration
|WB||Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa.
|ELISA||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.
1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts.
ab11003 staining Actin in Human SW480 cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed and then blocked using 10% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/50 for 2 hours at 25°C. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 568 (red) used at a 1/100 dilution.
Immunohistochemical analysis of frozen Human tongue tissue, using ab11003.
ab11003 staining Human fibroblast cells by ELISA (Sandwich-capture). Cells were blocked in 1% BSA for 1 hour at 25°C. The primary antibody ab11003 (Capture antibody) was diluted 1/3000 and incubated with sample for 12 hours at 4°C. ab5694 was used as secondary and a HRP conjugated goat polyclonal to mouse, diluted 1/1000 was used as detection antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"