Rat cardiac muscle tissue. Lysate from rat cardiac muscle tissue, rat brain tissue, rat testis tissue, rat skeletal muscle tissue. Whole cell lysate prepared from MM231 cells, HeLa cells, SMMC cells, HT1080 cells, SW620 cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 42 kDa.
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed. Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent. Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
All lanes : Anti-Actin antibody (ab95437) at 0.5 µg/ml
Lane 1 : Rat cardiac muscle missue lysate Lane 2 : Rat brain tissue lysate Lane 3 : Rat testis tissue lysate Lane 4 : Rat skeletal muscle tissue lysate Lane 5 : MM231 cell lysate Lane 6 : HeLa cell lysate Lane 7 : SMMC cell lysate Lane 8 : HT1080 cell lysate Lane 9 : SW620 cell lysate
Carpinelli MR et al. A new mouse model of Canavan leukodystrophy displays hearing impairment due to central nervous system dysmyelination. Dis Model Mech7:649-57 (2014).
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