Anti-ACSS1抗体(ab101570)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to ACSS1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Recombinant fragment, corresponding to internal sequence amino acids 368-629 of Human ACSS1 (BC039261).

  • 阳性对照
    • Human fetal brain lysate

性能

  • 形式
    Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG

应用

Our Abpromise guarantee covers the use of ab101570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).

靶标

  • 功能
    Important for maintaining normal body temperature during fasting and for energy homeostasis. Essential for energy expenditure under ketogenic conditions (By similarity). Converts acetate to acetyl-CoA so that it can be used for oxidation through the tricarboxylic cycle to produce ATP and CO(2).
  • 序列相似性
    Belongs to the ATP-dependent AMP-binding enzyme family.
  • 翻译后修饰
    Reversibly acetylated on Lys-642. The acetyl-CoA synthase activity is inhibited by acetylation and activated by deacetylation.
  • 细胞定位
    Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACAS2L antibody
    • AceCS2 antibody
    • AceCS2L antibody
    • Acetate CoA ligase 2 antibody
    • Acetate--CoA ligase 2 antibody
    • Acetyl CoA synthetase 2 antibody
    • Acetyl Coenzyme A synthetase 2 (AMP forming) like antibody
    • Acetyl coenzyme A synthetase 2 like, mitochondrial antibody
    • Acetyl-CoA synthetase 2 antibody
    • Acetyl-coenzyme A synthetase 2-like, mitochondrial antibody
    • ACS2L_HUMAN antibody
    • Acss1 antibody
    • Acyl CoA synthetase short chain family member 1 antibody
    • Acyl-CoA synthetase short-chain family member 1 antibody
    • FLJ45659 antibody
    • KIAA1846 antibody
    • MGC33843 antibody
    see all

Anti-ACSS1 antibody 图像

  • Anti-ACSS1 antibody (ab101570) at 1/500 dilution + Human fetal brain lysate

    Predicted band size : 75 kDa
    Observed band size : 75 kDa

实验方案

Anti-ACSS1 antibody (ab101570)参考文献

ab101570 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Rat Cell lysate - whole cell (Ovarian granulosa cells)
Specification
Ovarian granulosa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Dec 12 2013

Western blot

Excellent
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8)
Sample
Mouse Tissue lysate - whole (Heart)
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 22 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册