概述

  • 产品名称Anti-ACSL3抗体
    参阅全部 ACSL3 一抗
  • 描述
    兔多克隆抗体to ACSL3
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Orangutan
  • 免疫原

    Synthetic peptide: NSLDGLASVL YPGCDTLDKV FTYAKNKFKN KRLLGTREVL NEEDEVQPNG , corresponding to N terminal amino acids 72-121 of Human ACSL3

  • 阳性对照
    • 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab80675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis (By similarity). Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates (By similarity). Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins).
  • 序列相似性Belongs to the ATP-dependent AMP-binding enzyme family.
  • 细胞定位Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACS3 antibody
    • Acsl3 antibody
    • ACSL3_HUMAN antibody
    • Acyl CoA synthetase long chain family member 3 antibody
    • FACL3 antibody
    • Fatty acid Coenzyme A ligase long chain 3 antibody
    • LACS 3 antibody
    • LACS3 antibody
    • Lignoceroyl CoA synthase antibody
    • Long chain acyl CoA synthetase 3 antibody
    • Long chain fatty acid CoA ligase 3 antibody
    • Long-chain acyl-CoA synthetase 3 antibody
    • Long-chain-fatty-acid--CoA ligase 3 antibody
    • PRO2194 antibody
    see all

Anti-ACSL3 antibody 图像

  • Anti-ACSL3 antibody (ab80675) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 80 kDa
    Observed band size : 80 kDa
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 12%
  • Application: IHC. Species+tissue/cell type:THP-1 derived macrophage activated with iMtb. Primary antibody dilution: 1:200. Secondary antibody: Goat anti-rabbit Alexa Fluor 647. Secondary antibody dilution:1:333
  • Application: IHC. Species+tissue/cell type:THP-1 derived macrophage. Primary antibody dilution: 1:200. Secondary antibody: Goat anti-rabbit Alexa Fluor 647. Secondary antibody dilution:1:333

Anti-ACSL3 antibody (ab80675)参考文献

ab80675 has not yet been referenced specifically in any publications.

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