概述

  • 产品名称Anti-ACSBG1抗体[2B6]
    参阅全部 ACSBG1 一抗
  • 描述
    小鼠单克隆抗体[2B6] to ACSBG1
  • 经测试应用适用于: WB, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein of Human ACSBG1 produced in HEK293T cells (NP_055977).

  • 阳性对照
    • ACSBG1 transfected HEK293T cell lysate, Human colon, kidney, liver, Adenocarcinoma of ovary, and pancreas tissues
  • 常规说明Dilute in PBS (pH7.3) before use.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明ab118154 was purified from mouse ascites fluids by affinity chromatography.
  • 克隆单克隆
  • 克隆编号2B6
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab118154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 81 kDa.
IHC-P 1/50.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Mediates activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Able to activate long-chain fatty acids. Also able to activate very long-chain fatty acids; however, the relevance of such activity is unclear in vivo. Can activate diverse saturated, monosaturated and polyunsaturated fatty acids.
  • 组织特异性Expressed primarily in brain. Expressed at lower level in testis and adrenal gland. Present in all regions of brain except pituitary.
  • 序列相似性Belongs to the ATP-dependent AMP-binding enzyme family. Bubblegum subfamily.
  • 细胞定位Cytoplasm. Cytoplasmic vesicle. Microsome. Endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACBG1_HUMAN antibody
    • ACSBG 1 antibody
    • ACSBG1 antibody
    • Acyl CoA synthetase bubblegum family member 1 antibody
    • Acyl-CoA synthetase bubblegum family member 1 antibody
    • BG antibody
    • BG1 antibody
    • BGM antibody
    • Bubblegum antibody
    • FLJ30320 antibody
    • GR LACS antibody
    • GRLACS antibody
    • hBG1 antibody
    • hsBG antibody
    • hsBGM antibody
    • KIAA0631 antibody
    • Lipidosin antibody
    • Lipodisin antibody
    • Long chain fatty acid CoA ligase ACSBG1 antibody
    • Long-chain-fatty-acid--CoA ligase ACSBG1 antibody
    • LPD antibody
    • MGC14352 antibody
    • Ortholog of rodent gonadotropin regulated long chain acyl CoA synthetase antibody
    • Very long chain acyl CoA synthetase antibody
    see all

Anti-ACSBG1 antibody [2B6] 图像

  • Overlay histogram showing SH-SY5Y cells stained with ab118154 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab118154, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-ACSBG1 antibody [2B6] (ab118154) at 1/2000 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY ACSBG1 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 81 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC201652)
  • Immunohistochemical staining of paraffin-embedded Human colon tissue using ab118154 at a dilution of 1/50.
  • Immunohistochemical staining of paraffin-embedded Human kidney tissue using ab118154 at a dilution of 1/50.
  • Immunohistochemical staining of paraffin-embedded Human liver tissue using ab118154 at a dilution of 1/50.
  • Immunohistochemical staining of paraffin-embedded Human Adenocarcinoma of ovary tissue using ab118154 at a dilution of 1/50.
  • Immunohistochemical staining of paraffin-embedded Human pancreas tissue using ab118154 at a dilution of 1/50

Anti-ACSBG1 antibody [2B6] (ab118154)参考文献

This product has been referenced in:
  • Naka-Kaneda H  et al. The miR-17/106-p38 axis is a key regulator of the neurogenic-to-gliogenic transition in developing neural stem/progenitor cells. Proc Natl Acad Sci U S A 111:1604-9 (2014). Read more (PubMed: 24474786) »

See 1 Publication for this product

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