概述

  • 产品名称Anti-Acrosin抗体[ACR-2]
    参阅全部 Acrosin 一抗
  • 描述
    小鼠单克隆抗体[ACR-2] to Acrosin
  • 特异性The antibody ACR 2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa) in the acrosome of undamaged spermatozoa.
  • 经测试应用适用于: ICC, WBmore details
  • 种属反应性
    与反应: Pig
    不与反应: Cow, Dog, Human
  • 免疫原

    Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.

性能

应用

Our Abpromise guarantee covers the use of ab1900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC
WB
  • 应用说明ICC: Use at an assay dependent dilution membrane permeabilization (acetone) essential.
    WB: Use at an assay dependent dilution.

    The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa) in the acrosome of undamaged spermatozoa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.
    • 序列相似性Belongs to the peptidase S1 family.
      Contains 1 peptidase S1 domain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Acr antibody
      • ACRO_HUMAN antibody
      • Acrosin heavy chain antibody
      • Acrosin precursor antibody
      • ACRS antibody
      • Preproacrosin antibody
      • Proacrosin antibody
      see all

    Anti-Acrosin antibody [ACR-2] (ab1900)参考文献

    This product has been referenced in:
    • Moos J  et al. Protein-protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction. Biol Reprod 49:408-15 (1993). Read more (PubMed: 8373968) »
    • Moos J  et al. Relationship between molecular conversions of acrosin and the progression of exocytosis in the calcium ionophore-induced acrosome reaction. Biochim Biophys Acta 1176:199-207 (1993). Read more (PubMed: 8471622) »

    See all 4 Publications for this product

    Product Wall

    Thank you for your enquiry. Ab1900 has been tested for application in Immunocytochemistry and Western blotting, and yes, to use it in ICC membrane permeabilization (with acetone) is essential. I'm not aware of an antibody that would bind all sperm,...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"