• 产品名称Anti-ACRBP抗体
    参阅全部 ACRBP 一抗
  • 描述
    兔多克隆抗体to ACRBP
  • 经测试应用适用于: ICC/IF, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide derived from an internal region of human ACRBP.

  • 阳性对照
    • 293, HepG2 and Jurkat cells This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2



Our Abpromise guarantee covers the use of ab64809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
ELISA 1/10000.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • 功能May be involved in packaging and condensation of the acrosin zymogen in the acrosomal matrix via its association with proacrosin.
  • 组织特异性Expression restricted to testis in normal tissue. Expressed in a wide spectrum of cancers, including bladder, breast, liver, lung and colon cancers.
  • 翻译后修饰The N-terminus is blocked.
    Phosphorylated on Tyr residues in capacitated sperm.
  • 细胞定位Secreted. Cytoplasmic vesicle, secretory vesicle, acrosome. Colocalizes with proacrosin in the acrosome of sperm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACRBP antibody
    • ACRBP_HUMAN antibody
    • acrosin binding protein antibody
    • Acrosin binding protein precursor antibody
    • Acrosin-binding protein antibody
    • Cancer/testis antigen 23 antibody
    • Cancer/testis antigen OY TES 1 antibody
    • Cancer/testis antigen OY-TES-1 antibody
    • CT23 antibody
    • FLJ51160 antibody
    • OY TES 1 antibody
    • Proacrosin binding protein sp32 antibody
    • Proacrosin-binding protein sp32 antibody
    • SP32 antibody
    see all

Anti-ACRBP antibody 图像

  • ICC/IF image of ab64809 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab64809 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-ACRBP antibody (ab64809) at 1/500 dilution

    Lane 1 : Extracts of 293 cells
    Lane 2 : Extracts of HepG2 cells
    Lane 3 : Extracts of Jurkat cells
    Lane 4 : Extracts of Jurkat cells with immunizing Peptide

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 61 kDa
    Observed band size : 61 kDa
  • IHC image of ab64809 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64809, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-ACRBP antibody (ab64809)参考文献

ab64809 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 300000 cells
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (SKOV6 cells)
Specification SKOV6 cells
Blocking step Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C

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提交于 Mar 13 2014

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