Anti-ACRBP抗体(ab64809)
Key features and details
- Rabbit polyclonal to ACRBP
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ACRBP抗体 -
描述
兔多克隆抗体to ACRBP -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
A synthetic peptide derived from an internal region of human ACRBP.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab64809于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
May be involved in packaging and condensation of the acrosin zymogen in the acrosomal matrix via its association with proacrosin. -
组织特异性
Expression restricted to testis in normal tissue. Expressed in a wide spectrum of cancers, including bladder, breast, liver, lung and colon cancers. -
翻译后修饰
The N-terminus is blocked.
Phosphorylated on Tyr residues in capacitated sperm. -
细胞定位
Secreted. Cytoplasmic vesicle, secretory vesicle, acrosome. Colocalizes with proacrosin in the acrosome of sperm. - Information by UniProt
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数据库链接
- Entrez Gene: 84519 Human
- Omim: 608352 Human
- SwissProt: Q8NEB7 Human
- Unigene: 123239 Human
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别名
- ACRBP antibody
- ACRBP_HUMAN antibody
- acrosin binding protein antibody
see all
图片
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IHC image of ab64809 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64809, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
All lanes : Anti-ACRBP antibody (ab64809) at 1/500 dilution
Lane 1 : Extracts of 293 cells
Lane 2 : Extracts of HepG2 cells
Lane 3 : Extracts of Jurkat cells
Lane 4 : Extracts of Jurkat cells with immunizing Peptide
Lysates/proteins at 5 µg per lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab64809 被引用在 4 文献中.
- Lin L et al. Cancer-testis antigen ACRBP expression and serum immunoreactivity in ovarian cancer: Its association with prognosis. Immun Inflamm Dis 9:1759-1770 (2021). PubMed: 34528758
- Liu X et al. iTRAQ-based analysis of sperm proteome from normozoospermic men achieving the rescue-ICSI pregnancy after the IVF failure. Clin Proteomics 15:27 (2018). PubMed: 30166971
- Li X et al. Serum immunoreactivity of cancer/testis antigen OY-TES-1 and its tissues expression in glioma. Oncol Lett 13:3080-3086 (2017). PubMed: 28529561
- Cen YH et al. Knockdown of OY-TES-1 by RNAi causes cell cycle arrest and migration decrease in bone marrow-derived mesenchymal stem cells. Cell Biol Int 36:917-22 (2012). PubMed: 22651134