Anti-ACOX1抗体- Aminoterminal end (ab59964)


  • 产品名称Anti-ACOX1抗体- Aminoterminal end
    参阅全部 ACOX1 一抗
  • 描述
    兔多克隆抗体to ACOX1 - Aminoterminal end
  • 经测试应用适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide from the N-terminal region or human ACOX1 conjugated to KLH

  • 阳性对照
    • Mouse liver lysate



Our Abpromise guarantee covers the use of ab59964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/1000.
WB 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).


  • 功能Catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs. Isoform 2 is twice as active as isoform 1 against 16-hydroxy-palmitoyl-CoA and is 25% more active against 1,16-hexadecanodioyl-CoA.
  • 组织特异性Widely expressed with highest levels of isoform 1 and isoform 2 detected in testis. Isoform 1 is expressed at higher levels than isoform 2 in liver and kidney while isoform 2 levels are higher in brain, lung, muscle, white adipose tissue and testis. Levels are almost equal in heart.
  • 通路Lipid metabolism; peroxisomal fatty acid beta-oxidation.
  • 疾病相关Adrenoleukodystrophy, pseudoneonatal
  • 序列相似性Belongs to the acyl-CoA oxidase family.
  • 细胞定位Peroxisome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACOX antibody
    • ACOX1 antibody
    • ACOX1_HUMAN antibody
    • Acyl CoA oxidase 1 palmitoyl antibody
    • Acyl CoA oxidase straight chain antibody
    • AOX antibody
    • EC antibody
    • PALMCOX antibody
    • Palmitoyl CoA oxidase antibody
    • Palmitoyl-CoA oxidase antibody
    • Peroxisomal acyl coenzyme A oxidase 1 antibody
    • Peroxisomal acyl-coenzyme A oxidase 1 antibody
    • Peroxisomal fatty acyl CoA oxidase antibody
    • SCOX antibody
    • Straight chain acyl CoA oxidase antibody
    • Straight-chain acyl-CoA oxidase antibody
    see all

Anti-ACOX1 antibody - Aminoterminal end 图像

  • Anti-ACOX1 antibody - Aminoterminal end (ab59964) at 1/1000 dilution + Mouse liver lysate

    Predicted band size : 74 kDa
    Observed band size : 74 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • IHC image of ab59964 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab59964, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-ACOX1 antibody - Aminoterminal end (ab59964)参考文献

This product has been referenced in:
  • Le TT  et al. Uridine prevents fenofibrate-induced fatty liver. PLoS One 9:e87179 (2014). WB ; Mouse . Read more (PubMed: 24475249) »
  • Wu Y  et al. Differential effects of triclosan on the activation of mouse and human peroxisome proliferator-activated receptor alpha. Toxicol Lett 231:17-28 (2014). Read more (PubMed: 25193434) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Sample Mouse Tissue lysate - whole (Brain)
Specification Brain
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 May 26 2014