Anti-ACOX1抗体- Aminoterminal end (ab59964)

概述

  • 产品名称
    Anti-ACOX1抗体- Aminoterminal end
    参阅全部 ACOX1 一抗
  • 描述
    兔多克隆抗体to ACOX1 - Aminoterminal end
  • 经测试应用
    适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide from the N-terminal region or human ACOX1 conjugated to KLH

  • 阳性对照
    • Mouse liver lysate

性能

应用

Our Abpromise guarantee covers the use of ab59964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/1000.
WB 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).

靶标

  • 功能
    Catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs. Isoform 2 is twice as active as isoform 1 against 16-hydroxy-palmitoyl-CoA and is 25% more active against 1,16-hexadecanodioyl-CoA.
  • 组织特异性
    Widely expressed with highest levels of isoform 1 and isoform 2 detected in testis. Isoform 1 is expressed at higher levels than isoform 2 in liver and kidney while isoform 2 levels are higher in brain, lung, muscle, white adipose tissue and testis. Levels are almost equal in heart.
  • 通路
    Lipid metabolism; peroxisomal fatty acid beta-oxidation.
  • 疾病相关
    Adrenoleukodystrophy, pseudoneonatal
  • 序列相似性
    Belongs to the acyl-CoA oxidase family.
  • 细胞定位
    Peroxisome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACOX antibody
    • ACOX1 antibody
    • ACOX1_HUMAN antibody
    • Acyl CoA oxidase 1 palmitoyl antibody
    • Acyl CoA oxidase straight chain antibody
    • AOX antibody
    • EC 1.3.3.6 antibody
    • PALMCOX antibody
    • Palmitoyl CoA oxidase antibody
    • Palmitoyl-CoA oxidase antibody
    • Peroxisomal acyl coenzyme A oxidase 1 antibody
    • Peroxisomal acyl-coenzyme A oxidase 1 antibody
    • Peroxisomal fatty acyl CoA oxidase antibody
    • SCOX antibody
    • Straight chain acyl CoA oxidase antibody
    • Straight-chain acyl-CoA oxidase antibody
    see all

图片

  • Anti-ACOX1 antibody - Aminoterminal end (ab59964) at 1/1000 dilution + Mouse liver lysate

    Predicted band size : 74 kDa
    Observed band size : 74 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • IHC image of ab59964 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab59964, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

文献

This product has been referenced in:
  • Naudí A  et al. Region-specific vulnerability to lipid peroxidation and evidence of neuronal mechanisms for polyunsaturated fatty acid biosynthesis in the healthy adult human central nervous system. Biochim Biophys Acta 1862:485-495 (2017). WB ; Human . Read more (PubMed: 28185952) »
  • Della Casa L  et al. Effect of diets supplemented with different conjugated linoleic acid (CLA) isomers on protein expression in C57/BL6 mice. Genes Nutr 11:26 (2016). Read more (PubMed: 27713773) »

See all 6 Publications for this product

客户评价及客户问答

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Sample
Mouse Tissue lysate - whole (Brain)
Specification
Brain
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 May 26 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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